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Cytogenotoxicity and inflammatory response in liver of rats exposed to different doses of cannabis nano emulsions.
Pinto, Thiago Guedes; Dos Anjos Rosario, Barbara; de Moraes Malinverni, Andrea Cristina; Xavier, Rosana; Ferreira, Yasmin Alaby Martins; Pisani, Luciana Pellegrini; de Aquino, Pedro Everson Alexandre; de Barros Viana, Glauce Socorro; de Souza, Daniel Vitor; de Barros Viana, Milena; Ribeiro, Daniel Araki.
Afiliação
  • Pinto TG; Department of Biosciences, Institute of Health and Society, Federal University of São Paulo, UNIFESP, Rua Silva Jardim, 136, Room 332, Vila Mathias, Santos, SP, 11050-020, Brazil.
  • Dos Anjos Rosario B; Department of Biosciences, Institute of Health and Society, Federal University of São Paulo, UNIFESP, Rua Silva Jardim, 136, Room 332, Vila Mathias, Santos, SP, 11050-020, Brazil.
  • de Moraes Malinverni AC; Department of Pathology, Paulista Medical School, Federal University of Sao Paulo, UNIFESP, Sao Paulo, SP, Brazil.
  • Xavier R; Department of Pathology, Paulista Medical School, Federal University of Sao Paulo, UNIFESP, Sao Paulo, SP, Brazil.
  • Ferreira YAM; Department of Biosciences, Institute of Health and Society, Federal University of São Paulo, UNIFESP, Rua Silva Jardim, 136, Room 332, Vila Mathias, Santos, SP, 11050-020, Brazil.
  • Pisani LP; Department of Biosciences, Institute of Health and Society, Federal University of São Paulo, UNIFESP, Rua Silva Jardim, 136, Room 332, Vila Mathias, Santos, SP, 11050-020, Brazil.
  • de Aquino PEA; Department of Physiology and Pharmacology, Federal University of Ceara, UFC, Fortaleza, CE, Brazil.
  • de Barros Viana GS; Department of Physiology and Pharmacology, Federal University of Ceara, UFC, Fortaleza, CE, Brazil.
  • de Souza DV; Department of Biosciences, Institute of Health and Society, Federal University of São Paulo, UNIFESP, Rua Silva Jardim, 136, Room 332, Vila Mathias, Santos, SP, 11050-020, Brazil.
  • de Barros Viana M; Department of Biosciences, Institute of Health and Society, Federal University of São Paulo, UNIFESP, Rua Silva Jardim, 136, Room 332, Vila Mathias, Santos, SP, 11050-020, Brazil.
  • Ribeiro DA; Department of Biosciences, Institute of Health and Society, Federal University of São Paulo, UNIFESP, Rua Silva Jardim, 136, Room 332, Vila Mathias, Santos, SP, 11050-020, Brazil. daribeiro@unifesp.br.
Arch Toxicol ; 98(6): 1877-1890, 2024 Jun.
Article em En | MEDLINE | ID: mdl-38494580
ABSTRACT
Cannabis is the most used illicit substance for recreational purposes around the world. However, it has become increasingly common to witness the use of approved cannabis preparations for symptoms management in various diseases. The aim of this study was to investigate the effects of cannabis nano emulsion in the liver of Wistar rats, with different proportions of delta-9-tetrahydrocannabinol (THC) and cannabidiol (CBD). For this, a total of 40 male Wistar rats were distributed into 5 groups, as follows (n = 8 per group) Control G1, Experimental group (G2) treated with cannabis nano emulsion (THC and CBD) at a dose of 2.5 mg/kg, Experimental group (G3) treated with cannabis nano emulsion (THC and CBD) at a dose of 5 mg/kg, Experimental group (G4) treated with cannabis nano emulsion (CBD) at a dose of 2.5 mg/kg; Experimental group (G5) treated with cannabis nano emulsion (CBD) at a dose of 5 mg/kg. Exposure to the nano emulsion was carried out for 21 days, once a day, orally (gavage). Our results showed that cannabis nano emulsions at higher doses (5 mg/kg), regardless of the composition, induced histopathologic changes in the liver (G3 and G5) in comparison with the control group. In line with that, placental glutathione S-transferase (GST-P) positive foci increased in both G3 and G5 (p < 0.05), as well as the immune expression of Ki-67, vascular endothelial growth factor (VEGF) and p53 (p < 0.05). Also, the nano emulsion intake induced an increase in the number of micronucleated hepatocytes in G5 (p < 0.05) whereas G3 showed an increase in binucleated cells (p < 0.05). As for metanuclear alterations, karyolysis and pyknosis had an increased frequency in G3 (p < 0.05). Taken together, the results show that intake of cannabis nano emulsion may induce degenerative changes and genotoxicity in the liver in higher doses, demonstrating a clear dose-response relationship.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Canabidiol / Cannabis / Ratos Wistar / Relação Dose-Resposta a Droga / Emulsões / Fígado Limite: Animals Idioma: En Revista: Arch Toxicol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Canabidiol / Cannabis / Ratos Wistar / Relação Dose-Resposta a Droga / Emulsões / Fígado Limite: Animals Idioma: En Revista: Arch Toxicol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha