Your browser doesn't support javascript.
loading
Evaluation of the biological function of ribosomal protein S18 from cattle tick Rhipicephalus microplus.
Costa, Gabriel C A; Silva, Fernando A A; Torquato, Ricardo J S; Silva Vaz, Itabajara; Parizi, Luís F; Tanaka, Aparecida S.
Afiliação
  • Costa GCA; Departamento de Bioquímica, Escola Paulista de Medicina, Universidade de Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil.
  • Silva FAA; Departamento de Bioquímica, Escola Paulista de Medicina, Universidade de Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil.
  • Torquato RJS; Departamento de Bioquímica, Escola Paulista de Medicina, Universidade de Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil.
  • Silva Vaz I; Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), RS, Brazil; Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), RS, Brazil; Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular (INCT-EM), RJ, Brazil.
  • Parizi LF; Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), RS, Brazil; Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), RS, Brazil.
  • Tanaka AS; Departamento de Bioquímica, Escola Paulista de Medicina, Universidade de Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil; Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular (INCT-EM), RJ, Brazil. Electronic address: astanaka10@unifesp.br.
Ticks Tick Borne Dis ; 15(4): 102333, 2024 Jul.
Article em En | MEDLINE | ID: mdl-38522220
ABSTRACT
Rhipicephalus (Boophilus) microplus, also known as the cattle tick, causes severe parasitism and transmits different pathogens to vertebrate hosts, leading to massive economic losses. In the present study, we performed a functional characterization of a ribosomal protein from R. microplus to investigate its importance in blood feeding, egg production and viability. Ribosomal protein S18 (RPS18) is part of the 40S subunit, associated with 18S rRNA, and has been previously pointed to have a secondary role in different organisms. Rhipicephalus microplus RPS18 (RmRPS18) gene expression levels were modulated in female salivary glands during blood feeding. Moreover, mRNA levels in this tissue were 10 times higher than those in the midgut of fully engorged female ticks. Additionally, recombinant RmRPS18 was recognized by IgG antibodies from sera of cattle naturally or experimentally infested with ticks. RNAi-mediated knockdown of the RmRPS18 gene was performed in fully engorged females, leading to a significant (29 %) decrease in egg production. Additionally, egg hatching was completely impaired, suggesting that no viable eggs were produced by the RmRPS18-silenced group. Furthermore, antimicrobial assays revealed inhibitory activities against gram-negative Escherichia coli and gram-positive Staphylococcus aureus bacteria, affecting bacterial growth. Data presented here show the important role of RmRPS18 in tick physiology and suggest that RmRPS18 can be a potential target for the development of novel strategies for tick control.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Ribossômicas / Rhipicephalus / Proteínas de Artrópodes Limite: Animals Idioma: En Revista: Ticks Tick Borne Dis Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Ribossômicas / Rhipicephalus / Proteínas de Artrópodes Limite: Animals Idioma: En Revista: Ticks Tick Borne Dis Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda