Understanding the Structural Requirements of Peptide-Protein Interaction and Applications for Peptidomimetic Development.

Macias, Angy Liseth Davalos; Alecrim, Lilian Costa; Almeida, Fabio C L; Giordano, Ricardo Jose

Macias, Angy Liseth Davalos; Alecrim, Lilian Costa; Almeida, Fabio C L; Giordano, Ricardo Jose.

Afiliação

Macias ALD; Biochemistry Department, Institute of Chemistry, University of Sao Paulo, Sao Paulo, Brazil.
Alecrim LC; Biochemistry Department, Institute of Chemistry, University of Sao Paulo, Sao Paulo, Brazil.
Almeida FCL; Institute of Medical Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Giordano RJ; Biochemistry Department, Institute of Chemistry, University of Sao Paulo, Sao Paulo, Brazil. giordano@iq.usp.br.

Methods Mol Biol ; 2793: 65-82, 2024.

Article em En | MEDLINE | ID: mdl-38526724

ABSTRACT

ABSTRACT

Protein-protein interaction is at the heart of most biological processes, and small peptides that bind to protein binding sites are resourceful tools to explore and understand the structural requirements for these interactions. In that sense, phage display is a well-suited technology to study protein-protein interactions, as it allows for unbiased screening of billions of peptides in search for those that interact with a protein binding domain. Here, we will illustrate how two distinct but complementary approaches, phage display and nuclear magnetic resonance (NMR), can be utilized to unveil structural details of peptide-protein interaction. Finally, knowledge derived from phage mutagenesis and NMR studies can be streamlined for quick peptidomimetic design and synthesis using the retroinversion approach to validate using in vitro and in vivo assays the therapeutic potential of peptides identified by phage display.

Assuntos

Peptidomiméticos; Biblioteca de Peptídeos; Peptídeos/química; Proteínas/genética; Técnicas de Visualização da Superfície Celular

Palavras-chave

NMRNuclear magnetic resonance (NMR); Nuclear magnetic resonance; Peptide and protein interaction; Peptidomimetic design; Phage display; Phage mutagenesis

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptidomiméticos Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos

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