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Specific nanoprobe design for MRI: Targeting laminin in the blood-brain barrier to follow alteration due to neuroinflammation.
Zapata-Acevedo, Juan F; Losada-Barragán, Mónica; Osma, Johann F; Cruz, Juan C; Reiber, Andreas; Petry, Klaus G; Caillard, Amael; Sauldubois, Audrey; Llamosa Pérez, Daniel; Morillo Zárate, Aníbal José; Muñoz, Sonia Bermúdez; Daza Moreno, Agustín; Silva, Rafaela V; Infante-Duarte, Carmen; Chamorro-Coral, William; González-Reyes, Rodrigo E; Vargas-Sánchez, Karina.
Afiliação
  • Zapata-Acevedo JF; Grupo de Investigación en Neurociencias (NeURos), Centro de Neurociencia Neurovitae-UR, Instituto de Medicina Traslacional (IMT), Escuela de Medicina y Ciencias de la Salud, Universidad del Rosario, Bogotá, Colombia.
  • Losada-Barragán M; Grupo de Biología Celular y Funcional e Ingeniería de Biomoleculas, Departamento de Biología, Universidad Antonio Nariño, Bogotá, Colombia.
  • Osma JF; Department of Electrical and Electronic Engineering, Universidad de los Andes, Bogotá, Colombia.
  • Cruz JC; Department of Biomedical Engineering, Universidad de los Andes, Bogotá, Colombia.
  • Reiber A; Department of Biomedical Engineering, Universidad de los Andes, Bogotá, Colombia.
  • Petry KG; Chemistry Department, Grupo La Quimica en la interfase inorgánica-orgánica QUINORG, Universidad de los Andes, Bogotá, Colombia.
  • Caillard A; CNRS UMR 5536 Centre de Resonance Magnétique des Systemes Biologiques and INSERM U1049 Neuroinflammation, University of Bordeaux, Bordeaux, France.
  • Sauldubois A; GREMI, UMR7344, University of Orleans, Orleans, France.
  • Llamosa Pérez D; GREMI, UMR7344, University of Orleans, Orleans, France.
  • Morillo Zárate AJ; Facultad de Ciencias, Grupo Investigación fundamental y aplicada en Materiales, Universidad Antonio Nariño, Bogotá, Colombia.
  • Muñoz SB; Médico Radiólogo Institucional, Fundación Santa Fé de Bogotá, Bogotá, Colombia.
  • Daza Moreno A; Médico Radiólogo Institucional, Fundación Santa Fé de Bogotá, Bogotá, Colombia.
  • Silva RV; Oficial de Protección Radiológica, Fundación Santa Fé de Bogotá, Bogotá, Colombia.
  • Infante-Duarte C; Experimental and Clinical Research Center, a Cooperation between the Max Delbrück Center for Molecular Medicine in the Helmholtz Association and Charité-Universitätsmedizin Berlin, Berlin, Germany.
  • Chamorro-Coral W; Experimental and Clinical Research Center, a Cooperation between the Max Delbrück Center for Molecular Medicine in the Helmholtz Association and Charité-Universitätsmedizin Berlin, Berlin, Germany.
  • González-Reyes RE; Laboratorio de Neurofisiología Celular, Grupo de Neurociencia Traslacional, Facultad de Medicina, Universidad de los Andes, Bogotá, Colombia.
  • Vargas-Sánchez K; Grupo de Investigación en Neurociencias (NeURos), Centro de Neurociencia Neurovitae-UR, Instituto de Medicina Traslacional (IMT), Escuela de Medicina y Ciencias de la Salud, Universidad del Rosario, Bogotá, Colombia.
PLoS One ; 19(4): e0302031, 2024.
Article em En | MEDLINE | ID: mdl-38603692
ABSTRACT
Chronic neuroinflammation is characterized by increased blood-brain barrier (BBB) permeability, leading to molecular changes in the central nervous system that can be explored with biomarkers of active neuroinflammatory processes. Magnetic resonance imaging (MRI) has contributed to detecting lesions and permeability of the BBB. Ultra-small superparamagnetic particles of iron oxide (USPIO) are used as contrast agents to improve MRI observations. Therefore, we validate the interaction of peptide-88 with laminin, vectorized on USPIO, to explore BBB molecular alterations occurring during neuroinflammation as a potential tool for use in MRI. The specific labeling of NPS-P88 was verified in endothelial cells (hCMEC/D3) and astrocytes (T98G) under inflammation induced by interleukin 1ß (IL-1ß) for 3 and 24 hours. IL-1ß for 3 hours in hCMEC/D3 cells increased their co-localization with NPS-P88, compared with controls. At 24 hours, no significant differences were observed between groups. In T98G cells, NPS-P88 showed similar nonspecific labeling among treatments. These results indicate that NPS-P88 has a higher affinity towards brain endothelial cells than astrocytes under inflammation. This affinity decreases over time with reduced laminin expression. In vivo results suggest that following a 30-minute post-injection, there is an increased presence of NPS-P88 in the blood and brain, diminishing over time. Lastly, EAE animals displayed a significant accumulation of NPS-P88 in MRI, primarily in the cortex, attributed to inflammation and disruption of the BBB. Altogether, these results revealed NPS-P88 as a biomarker to evaluate changes in the BBB due to neuroinflammation by MRI in biological models targeting laminin.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Barreira Hematoencefálica / Laminina Limite: Animals Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Barreira Hematoencefálica / Laminina Limite: Animals Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Estados Unidos