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ß-Adrenergic Stimulation-Induced PVAT Dysfunction in Male Sex: A Role for 11ß-Hydroxysteroid Dehydrogenase-1.
Victorio, Jamaira Aparecida; Barssotti, Letícia; Aprahamian, Tamar; Costa, Raul Gobato; Mousovich-Neto, Felippe; Oliveira, Helena Coutinho Franco; Mori, Marcelo; Rossoni, Luciana Venturini; Davel, Ana Paula.
Afiliação
  • Victorio JA; Laboratory of Vascular Biology (LaBiVasc), Department of Structural and Functional Biology, Universidade Estadual de Campinas (UNICAMP), São Paulo 13083-865, Brazil.
  • Barssotti L; Laboratory of Vascular Biology (LaBiVasc), Department of Structural and Functional Biology, Universidade Estadual de Campinas (UNICAMP), São Paulo 13083-865, Brazil.
  • Aprahamian T; Department of Medicine - Renal Section, Boston University School of Medicine, Boston, MA 02118, USA.
  • Costa RG; Department of Biochemistry and Tissue Biology, Universidade Estadual de Campinas (UNICAMP), São Paulo 13083-865, Brazil.
  • Mousovich-Neto F; Department of Biochemistry and Tissue Biology, Universidade Estadual de Campinas (UNICAMP), São Paulo 13083-865, Brazil.
  • Oliveira HCF; Obesity and Comorbidities Research Center, Universidade Estadual de Campinas (UNICAMP), São Paulo 13083-865, Brazil.
  • Mori M; Department of Biochemistry and Tissue Biology, Universidade Estadual de Campinas (UNICAMP), São Paulo 13083-865, Brazil.
  • Rossoni LV; Obesity and Comorbidities Research Center, Universidade Estadual de Campinas (UNICAMP), São Paulo 13083-865, Brazil.
  • Davel AP; Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo 05508-000, Brazil.
Endocrinology ; 165(6)2024 Apr 29.
Article em En | MEDLINE | ID: mdl-38712392
ABSTRACT
Long-term ß-adrenoceptor (ß-AR) stimulation is a pathological mechanism associated with cardiovascular diseases resulting in endothelial and perivascular adipose tissue (PVAT) dysfunction. In this study, we aimed to identify whether ß-adrenergic signaling has a direct effect on PVAT. Thoracic aorta PVAT was obtained from male Wistar rats and cultured ex vivo with the ß-AR agonist isoproterenol (Iso; 1 µM) or vehicle for 24 hours. Conditioned culture medium (CCM) from Iso-treated PVAT induced a marked increase in aorta contractile response, induced oxidative stress, and reduced nitric oxide production in PVAT compared to vehicle. In addition, Iso-treated PVAT and PVAT-derived differentiated adipocytes exhibited higher corticosterone release and protein expression of 11ß-hydroxysteroid dehydrogenase type 1 (11ß-HSD1), an enzyme responsible for de novo synthesis of corticosterone. Macrophages exposed to Iso also exhibited increased corticosterone release in response to ß-AR stimulation. Incubation of Iso-treated PVAT and PVAT-derived differentiated adipocytes with ß3-AR antagonist restored aorta contractile function modulated by Iso-CCM and normalized 11ß-HSD1 protein expression. These results show that ß3-AR signaling leads to upregulation of 11ß-HSD1 in PVAT, thus increasing corticosterone release and contributing to impair the anticontractile function of this tissue.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Corticosterona / 11-beta-Hidroxiesteroide Desidrogenase Tipo 1 / Isoproterenol Limite: Animals Idioma: En Revista: Endocrinology Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Corticosterona / 11-beta-Hidroxiesteroide Desidrogenase Tipo 1 / Isoproterenol Limite: Animals Idioma: En Revista: Endocrinology Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos