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AMPK inhibits voltage-gated calcium channel-current in rat chromaffin cells.
Fukumoto-Inukai, A K; Bermeo, K; Arenas, I; Rosendo-Pineda, M J; Pimentel-Cabrera, J A; Garcia, D E.
Afiliação
  • Fukumoto-Inukai AK; Departamento de Fisiología, Facultad de Medicina, Universidad Nacional Autónoma de México, UNAM, Circuito Exterior S/N, Ciudad Universitaria, Coyoacán, 04510, Ciudad de México, Mexico.
  • Bermeo K; Licenciatura en Neurociencias, Facultad de Medicina, Universidad Nacional Autónoma de México, UNAM, Circuito Exterior S/N, Ciudad Universitaria, Coyoacán, 04510, Ciudad de México, Mexico.
  • Arenas I; Departamento de Fisiología, Facultad de Medicina, Universidad Nacional Autónoma de México, UNAM, Circuito Exterior S/N, Ciudad Universitaria, Coyoacán, 04510, Ciudad de México, Mexico.
  • Rosendo-Pineda MJ; Departamento de Fisiología, Facultad de Medicina, Universidad Nacional Autónoma de México, UNAM, Circuito Exterior S/N, Ciudad Universitaria, Coyoacán, 04510, Ciudad de México, Mexico.
  • Pimentel-Cabrera JA; Laboratorio Nacional de Microscopía Avanzada, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico.
  • Garcia DE; Departamento de Fisiología, Facultad de Medicina, Universidad Nacional Autónoma de México, UNAM, Circuito Exterior S/N, Ciudad Universitaria, Coyoacán, 04510, Ciudad de México, Mexico. Electronic address: erasmo@unam.mx.
Mol Cell Endocrinol ; 591: 112275, 2024 Sep 15.
Article em En | MEDLINE | ID: mdl-38777212
ABSTRACT
Metabolic changes are critical in the regulation of Ca2+ influx in central and peripheral neuroendocrine cells. To study the regulation of L-type Ca2+ channels by AMPK we used biochemical reagents and ATP/glucose-concentration manipulations in rat chromaffin cells. AICAR and Compound-C, at low concentration, significantly induce changes in L-type Ca2+ channel-current amplitude and voltage dependence. Remarkably, an overlasting decrease in the channel-current density can be induced by lowering the intracellular level of ATP. Accordingly, Ca2+ channel-current density gradually diminishes by decreasing the extracellular glucose concentration. By using immunofluorescence, a decrease in the expression of CaV1.2 is observed while decreasing extracellular glucose, suggesting that AMPK reduces the number of functional Ca2+ channels into the plasma membrane. Together, these results support for the first time the dependence of metabolic changes in the maintenance of Ca2+ channel-current by AMPK. They reveal a key step in Ca2+ influx in secretory cells.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células Cromafins / Canais de Cálcio Tipo L / Proteínas Quinases Ativadas por AMP / Glucose / Aminoimidazol Carboxamida Limite: Animals Idioma: En Revista: Mol Cell Endocrinol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: México País de publicação: Irlanda
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células Cromafins / Canais de Cálcio Tipo L / Proteínas Quinases Ativadas por AMP / Glucose / Aminoimidazol Carboxamida Limite: Animals Idioma: En Revista: Mol Cell Endocrinol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: México País de publicação: Irlanda