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Tissue-specific differences in Ca2+ sensitivity of the mitochondrial permeability transition pore (PTP). Experiments in male rat liver and heart.
Ricardez-Garcia, Carolina; Reyes-Becerril, Mauricio; Mosqueda-Martinez, Edson; Mendez-Romero, Ofelia; Ruiz-Ramírez, Angelica; Uribe-Carvajal, Salvador.
Afiliação
  • Ricardez-Garcia C; Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad Universitaria, Mexico City, Mexico.
  • Reyes-Becerril M; Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad Universitaria, Mexico City, Mexico.
  • Mosqueda-Martinez E; Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad Universitaria, Mexico City, Mexico.
  • Mendez-Romero O; Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad Universitaria, Mexico City, Mexico.
  • Ruiz-Ramírez A; Departamento de Biomedicina Cardiovascular, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, Mexico.
  • Uribe-Carvajal S; Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad Universitaria, Mexico City, Mexico.
Physiol Rep ; 12(10): e16056, 2024 May.
Article em En | MEDLINE | ID: mdl-38777811
ABSTRACT
Permeability transition pore (PTP) opening dissipates ion and electron gradients across the internal mitochondrial membrane (IMM), including excess Ca2+ in the mitochondrial matrix. After opening, immediate PTP closure must follow to prevent outer membrane disruption, loss of cytochrome c, and eventual apoptosis. Flickering, defined as the rapid alternative opening/closing of PTP, has been reported in heart, which undergoes frequent, large variations in Ca2+. In contrast, in tissues that undergo depolarization events less often, such as the liver, PTP would not need to be as dynamic and thus these tissues would not be as resistant to stress. To evaluate this idea, it was decided to follow the reversibility of the permeability transition (PT) in isolated murine mitochondria from two different tissues the very dynamic heart, and the liver, which suffers depolarizations less frequently. It was observed that in heart mitochondria PT remained reversible for longer periods and at higher Ca2+ loads than in liver mitochondria. In all cases, Ca2+ uptake was inhibited by ruthenium red and PT was delayed by Cyclosporine A. Characterization of this phenomenon included measuring the rate of oxygen consumption, organelle swelling and Ca2+ uptake and retention. Results strongly suggest that there are tissue-specific differences in PTP physiology, as it resists many more Ca2+ additions before opening in a highly active organ such as the heart than in an organ that seldom suffers Ca2+ loading, such as the liver.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Mitocôndrias Hepáticas / Cálcio / Ratos Wistar / Proteínas de Transporte da Membrana Mitocondrial / Poro de Transição de Permeabilidade Mitocondrial / Mitocôndrias Cardíacas Limite: Animals Idioma: En Revista: Physiol Rep Ano de publicação: 2024 Tipo de documento: Article País de afiliação: México País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Mitocôndrias Hepáticas / Cálcio / Ratos Wistar / Proteínas de Transporte da Membrana Mitocondrial / Poro de Transição de Permeabilidade Mitocondrial / Mitocôndrias Cardíacas Limite: Animals Idioma: En Revista: Physiol Rep Ano de publicação: 2024 Tipo de documento: Article País de afiliação: México País de publicação: Estados Unidos