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Exploration of mobile genetic elements in the ruminal microbiome of Nellore cattle.
Faleiros, Camila A; Nunes, Alanne T; Gonçalves, Osiel S; Alexandre, Pâmela A; Poleti, Mirele D; Mattos, Elisângela C; Perna-Junior, Flavio; Rodrigues, Paulo H Mazza; Fukumasu, Heidge.
Afiliação
  • Faleiros CA; Department of Veterinary Medicine, School of Animal Science and Food Engineering (FZEA), University of São Paulo, Pirassununga, SP, 13635-900, Brazil.
  • Nunes AT; Department of Veterinary Medicine, School of Animal Science and Food Engineering (FZEA), University of São Paulo, Pirassununga, SP, 13635-900, Brazil.
  • Gonçalves OS; Department of Microbiology, Institute of Biotechnology Applied to Agriculture (BIOAGRO), Federal University of Viçosa, Viçosa, MG, 36570-000, Brazil.
  • Alexandre PA; Commonwealth Scientific and Industrial Research Organization (CSIRO), Agriculture and Food, Brisbane, QLD, Australia.
  • Poleti MD; Department of Veterinary Medicine, School of Animal Science and Food Engineering (FZEA), University of São Paulo, Pirassununga, SP, 13635-900, Brazil.
  • Mattos EC; Department of Veterinary Medicine, School of Animal Science and Food Engineering (FZEA), University of São Paulo, Pirassununga, SP, 13635-900, Brazil.
  • Perna-Junior F; Department of Animal Nutrition and Production, School of Veterinary Medicine and Animal Science, University of São Paulo (FMVZ-USP), Pirassununga, São Paulo, 13635-900, Brazil.
  • Rodrigues PHM; Department of Animal Nutrition and Production, School of Veterinary Medicine and Animal Science, University of São Paulo (FMVZ-USP), Pirassununga, São Paulo, 13635-900, Brazil.
  • Fukumasu H; Department of Veterinary Medicine, School of Animal Science and Food Engineering (FZEA), University of São Paulo, Pirassununga, SP, 13635-900, Brazil. fukumasu@usp.br.
Sci Rep ; 14(1): 13056, 2024 06 06.
Article em En | MEDLINE | ID: mdl-38844487
ABSTRACT
Metagenomics has made it feasible to elucidate the intricacies of the ruminal microbiome and its role in the differentiation of animal production phenotypes of significance. The search for mobile genetic elements (MGEs) has taken on great importance, as they play a critical role in the transfer of genetic material between organisms. Furthermore, these elements serve a dual purpose by controlling populations through lytic bacteriophages, thereby maintaining ecological equilibrium and driving the evolutionary progress of host microorganisms. In this study, we aimed to identify the association between ruminal bacteria and their MGEs in Nellore cattle using physical chromosomal links through the Hi-C method. Shotgun metagenomic sequencing and the proximity ligation method ProxiMeta were used to analyze DNA, getting 1,713,111,307 bp, which gave rise to 107 metagenome-assembled genomes from rumen samples of four Nellore cows maintained on pasture. Taxonomic analysis revealed that most of the bacterial genomes belonged to the families Lachnospiraceae, Bacteroidaceae, Ruminococcaceae, Saccharofermentanaceae, and Treponemataceae and mostly encoded pathways for central carbon and other carbohydrate metabolisms. A total of 31 associations between host bacteria and MGE were identified, including 17 links to viruses and 14 links to plasmids. Additionally, we found 12 antibiotic resistance genes. To our knowledge, this is the first study in Brazilian cattle that connect MGEs with their microbial hosts. It identifies MGEs present in the rumen of pasture-raised Nellore cattle, offering insights that could advance biotechnology for food digestion and improve ruminant performance in production systems.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Rúmen / Sequências Repetitivas Dispersas Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Rúmen / Sequências Repetitivas Dispersas Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido