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Utilization of Fusarium Solani lipase for enrichment of polyunsaturated Omega-3 fatty acids.
de Carvalho Silva, Allysson Kayron; Lima, Fernanda Jeniffer Lindoso; Borges, Katia Regina Assunção; Wolff, Laís Araújo Souza; de Andrade, Marcelo Souza; Alves, Rita de Nazaré Silva; Cordeiro, Carolina Borges; da Silva, Marcos Antônio Custódio Neto; Nascimento, Maria do Desterro Soares Brandão; da Silva Espósito, Talita; de Barros Bezerra, Geusa Felipa.
Afiliação
  • de Carvalho Silva AK; Doctoral Program in Biotechnology- Northeast Biotechnology Network (RENORBIO), Center for Basic and Applied Immunology (NIBA), Federal University of Maranhão, São Luís, Maranhão, Brazil. allyssonkayron@hotmail.com.
  • Lima FJL; Doctoral Program in Biotechnology- Northeast Biotechnology Network (RENORBIO), Center for Basic and Applied Immunology (NIBA), Federal University of Maranhão, São Luís, Maranhão, Brazil.
  • Borges KRA; Doctoral Program in Biotechnology- Northeast Biotechnology Network (RENORBIO), Center for Basic and Applied Immunology (NIBA), Federal University of Maranhão, São Luís, Maranhão, Brazil.
  • Wolff LAS; Postgraduate Program in Adult Health (PPGSAD), Center for Basic and Applied Immunology (NIBA), Federal University of Maranhão, São Luís, Maranhão, Brazil.
  • de Andrade MS; Postgraduate Program in Adult Health (PPGSAD), Center for Basic and Applied Immunology (NIBA), Federal University of Maranhão, São Luís, Maranhão, Brazil.
  • Alves RNS; Postgraduate Program in Adult Health (PPGSAD), Center for Basic and Applied Immunology (NIBA), Federal University of Maranhão, São Luís, Maranhão, Brazil.
  • Cordeiro CB; Postgraduate Program in Adult Health (PPGSAD), Center for Basic and Applied Immunology (NIBA), Federal University of Maranhão, São Luís, Maranhão, Brazil.
  • da Silva MACN; Departament of Medicina I, Federal University of Maranhão, Imperatriz, Maranhão, Brazil.
  • Nascimento MDDSB; Doctoral Program in Biotechnology- Northeast Biotechnology Network (RENORBIO), Center for Basic and Applied Immunology (NIBA), Federal University of Maranhão, São Luís, Maranhão, Brazil.
  • da Silva Espósito T; Postgraduate Program in Adult Health (PPGSAD), Center for Basic and Applied Immunology (NIBA), Federal University of Maranhão, São Luís, Maranhão, Brazil.
  • de Barros Bezerra GF; Department of Oceanography and Limnology, Laboratory of Biotechnology of Aquatic Organisms (BIOAQUA), Federal University of Maranhão, São Luís, Maranhão, Brazil.
Braz J Microbiol ; 55(3): 2211-2226, 2024 Sep.
Article em En | MEDLINE | ID: mdl-38874742
ABSTRACT
Omega-3 fatty acids, such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), offer numerous health benefits. Enriching these fatty acids in fish oil using cost-effective methods, like lipase application, has been studied extensively. This research aimed to investigate F. solani as a potential lipase producer and compare its efficacy in enhancing polyunsaturated omega-3 fatty acids with commercial lipases. Submerged fermentation with coconut oil yielded Lipase F2, showing remarkable activity (215.68 U/mL). Lipase F2 remained stable at pH 8.0 (activity 93.84 U/mL) and active between 35 and 70 °C, with optimal stability at 35 °C. It exhibited resistance to various surfactants and ions, showing no cytotoxic activity in vitro, crucial for its application in the food and pharmaceutical industries. Lipase F2 efficiently enriched EPA and DHA in fish oil, reaching 22.1 mol% DHA and 23.8 mol% EPA. These results underscore the economic viability and efficacy of Lipase F2, a partially purified enzyme obtained using low-cost techniques, demonstrating remarkable stability and resistance to diverse conditions. Its performance was comparable to highly pure commercially available enzymes in omega-3 production. These findings highlight the potential of F. solani as a promising lipase source, offering opportunities for economically producing omega-3 and advancing biotechnological applications in the food and supplements industry.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ácidos Graxos Ômega-3 / Fusarium / Lipase Idioma: En Revista: Braz J Microbiol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ácidos Graxos Ômega-3 / Fusarium / Lipase Idioma: En Revista: Braz J Microbiol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil