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Wild Patagonian yeast improve the evolutionary potential of novel interspecific hybrid strains for lager brewing.
Molinet, Jennifer; Navarrete, Juan P; Villarroel, Carlos A; Villarreal, Pablo; Sandoval, Felipe I; Nespolo, Roberto F; Stelkens, Rike; Cubillos, Francisco A.
Afiliação
  • Molinet J; ANID-Millennium Science Initiative-Millennium Institute for Integrative Biology (iBio), Santiago, Chile.
  • Navarrete JP; Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, Chile.
  • Villarroel CA; Department of Zoology, Stockholm University, Stockholm, Sweden.
  • Villarreal P; ANID-Millennium Science Initiative-Millennium Institute for Integrative Biology (iBio), Santiago, Chile.
  • Sandoval FI; ANID-Millennium Science Initiative-Millennium Institute for Integrative Biology (iBio), Santiago, Chile.
  • Nespolo RF; Centro de Biotecnología de los Recursos Naturales (CENBio), Facultad de Ciencias Agrarias y Forestales, Universidad Católica del Maule, Talca, Chile.
  • Stelkens R; ANID-Millennium Science Initiative-Millennium Institute for Integrative Biology (iBio), Santiago, Chile.
  • Cubillos FA; Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, Chile.
PLoS Genet ; 20(6): e1011154, 2024 Jun.
Article em En | MEDLINE | ID: mdl-38900713
ABSTRACT
Lager yeasts are limited to a few strains worldwide, imposing restrictions on flavour and aroma diversity and hindering our understanding of the complex evolutionary mechanisms during yeast domestication. The recent finding of diverse S. eubayanus lineages from Patagonia offers potential for generating new lager yeasts with different flavour profiles. Here, we leverage the natural genetic diversity of S. eubayanus and expand the lager yeast repertoire by including three distinct Patagonian S. eubayanus lineages. We used experimental evolution and selection on desirable traits to enhance the fermentation profiles of novel S. cerevisiae x S. eubayanus hybrids. Our analyses reveal an intricate interplay of pre-existing diversity, selection on species-specific mitochondria, de-novo mutations, and gene copy variations in sugar metabolism genes, resulting in high ethanol production and unique aroma profiles. Hybrids with S. eubayanus mitochondria exhibited greater evolutionary potential and superior fitness post-evolution, analogous to commercial lager hybrids. Using genome-wide screens of the parental subgenomes, we identified genetic changes in IRA2, IMA1, and MALX genes that influence maltose metabolism, and increase glycolytic flux and sugar consumption in the evolved hybrids. Functional validation and transcriptome analyses confirmed increased maltose-related gene expression, influencing greater maltotriose consumption in evolved hybrids. This study demonstrates the potential for generating industrially viable lager yeast hybrids from wild Patagonian strains. Our hybridization, evolution, and mitochondrial selection approach produced hybrids with high fermentation capacity and expands lager beer brewing options.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Saccharomyces cerevisiae / Cerveja / Fermentação / Hibridização Genética Idioma: En Revista: PLoS Genet Assunto da revista: GENETICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Chile País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Saccharomyces cerevisiae / Cerveja / Fermentação / Hibridização Genética Idioma: En Revista: PLoS Genet Assunto da revista: GENETICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Chile País de publicação: Estados Unidos