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Preparative Fractionation of Brazilian Red Propolis Extract Using Step-Gradient Counter-Current Chromatography.
Lopez, Begoña Gimenez-Cassina; Marcucci, Maria Cristina; Rocco, Silvana Aparecida; Sforça, Maurício Luís; Eberlin, Marcos Nogueira; Hewitson, Peter; Ignatova, Svetlana; Sawaya, Alexandra Christine Helena Frankland.
Afiliação
  • Lopez BG; Faculty of Pharmaceutical Science, State University of Campinas (UNICAMP), Campinas 13083-871, SP, Brazil.
  • Marcucci MC; Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University (ICT-UNESP), São José dos Campos 12245-000, SP, Brazil.
  • Rocco SA; Brazilian Biosciences National Laboratory (LNBio), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas 13083-970, SP, Brazil.
  • Sforça ML; Brazilian Biosciences National Laboratory (LNBio), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas 13083-970, SP, Brazil.
  • Eberlin MN; MACKGRAPHE-School of Engineering, Mackenzie Presbyterian University, São Paulo 01302-907, SP, Brazil.
  • Hewitson P; Department of Chemical Engineering, College of Engineering, Design and Physical Sciences (CEDPS), Brunel University London, Uxbridge UB8 3PH, UK.
  • Ignatova S; Department of Chemical Engineering, College of Engineering, Design and Physical Sciences (CEDPS), Brunel University London, Uxbridge UB8 3PH, UK.
  • Sawaya ACHF; Faculty of Pharmaceutical Science, State University of Campinas (UNICAMP), Campinas 13083-871, SP, Brazil.
Molecules ; 29(12)2024 Jun 09.
Article em En | MEDLINE | ID: mdl-38930823
ABSTRACT
Propolis is a resinous bee product with a very complex composition, which is dependent upon the plant sources that bees visit. Due to the promising antimicrobial activities of red Brazilian propolis, it is paramount to identify the compounds responsible for it, which, in most of the cases, are not commercially available. The aim of this study was to develop a quick and clean preparative-scale methodology for preparing fractions of red propolis directly from a complex crude ethanol extract by combining the extractive capacity of counter-current chromatography (CCC) with preparative HPLC. The CCC method development included step gradient elution for the removal of waxes (which can bind to and block HPLC columns), sample injection in a single solvent to improve stationary phase stability, and a change in the mobile phase flow pattern, resulting in the loading of 2.5 g of the Brazilian red propolis crude extract on a 912.5 mL Midi CCC column. Three compounds were subsequently isolated from the concentrated fractions by preparative HPLC and identified by NMR and high-resolution MS red pigment, retusapurpurin A; the isoflavan 3(R)-7-O-methylvestitol; and the prenylated benzophenone isomers xanthochymol/isoxanthochymol. These compounds are markers of red propolis that contribute to its therapeutic properties, and the amount isolated allows for further biological activities testing and for their use as chromatographic standards.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Própole / Distribuição Contracorrente Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Molecules Assunto da revista: BIOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Própole / Distribuição Contracorrente Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Molecules Assunto da revista: BIOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça