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Influence of Major Polyphenols on the Anti-Candida Activity of Eugenia uniflora Leaves: Isolation, LC-ESI-HRMS/MS Characterization and In Vitro Evaluation.
Tenório, Camylla Janiele Lucas; Dantas, Thainá Dos Santos; Abreu, Lucas Silva; Ferreira, Magda Rhayanny Assunção; Soares, Luiz Alberto Lira.
Afiliação
  • Tenório CJL; Laboratory of Pharmacognosy, Department of Pharmaceutical Sciences, Federal University of Pernambuco, Recife 50670-901, PE, Brazil.
  • Dantas TDS; Post-Graduate Program in Pharmaceutical Sciences, Federal University of Pernambuco, Recife 50670-901, PE, Brazil.
  • Abreu LS; Laboratory of Pharmacognosy, Department of Pharmaceutical Sciences, Federal University of Pernambuco, Recife 50670-901, PE, Brazil.
  • Ferreira MRA; Post-Graduate Program in Therapeutic Innovation, Federal University of Pernambuco, Recife 50670-901, PE, Brazil.
  • Soares LAL; Chemistry Institute, Fluminense Federal University, Niterói 24020-150, RJ, Brazil.
Molecules ; 29(12)2024 Jun 10.
Article em En | MEDLINE | ID: mdl-38930827
ABSTRACT
The content of chemical constituents in Eugenia uniflora leaf extracts correlates positively with biological activities. The experimental objective was to carry out the phytochemical screening and purification of the major polyphenols from the leaves of E. uniflora. In addition, the anti-Candida activity of the hydroalcoholic extract, fraction, subfractions and polyphenols purified were evaluated. After partitioning of the extract with ethyl acetate, the fractions were chromatographed on Sephadex® LH-20 gel followed by RP-flash chromatography and monitored by TLC and RP-HPLC. The samples were characterized by mass spectrometry (LC-ESI-QTOF-MS2) and subjected to the microdilution method in 96-well plates against strains of C. albicans, C. auris, and C. glabrata. Myricitrin (93.89%; w/w; m/z 463.0876), gallic acid (99.9%; w/w; m/z 169.0142), and ellagic acid (94.2%; w/w; m/z 300.9988) were recovered. The polyphenolic fraction (62.67% (w/w) myricitrin) and the ellagic fraction (67.86% (w/w) ellagic acid) showed the best antifungal performance (MIC between 62.50 and 500 µg/mL), suggesting an association between the majority constituents and the antifungal response of E. uniflora derivatives. However, there is a clear dependence on the presence of the complex chemical mixture. In conclusion, chromatographic strategies were effectively employed to recover the major polyphenols from the leaves of the species.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Extratos Vegetais / Folhas de Planta / Polifenóis / Eugenia / Antifúngicos Idioma: En Revista: Molecules Assunto da revista: BIOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Extratos Vegetais / Folhas de Planta / Polifenóis / Eugenia / Antifúngicos Idioma: En Revista: Molecules Assunto da revista: BIOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça