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Exploring xylose metabolism in non-conventional yeasts: kinetic characterization and product accumulation under different aeration conditions.
Bolzico, Bruna C; Racca, Sofia; Khawam, Jorge N; Leonardi, Rodrigo J; Tomassi, Ariel H; Benzzo, Maria T; Comelli, Raul N.
Afiliação
  • Bolzico BC; Grupo de Procesos Biológicos en Ingeniería Ambiental (GPBIA), Facultad de Ingeniería y Ciencias Hídricas (FICH), Universidad Nacional del Litoral (UNL), Ciudad Universitaria, CC 242 Paraje El Pozo, Santa Fe 3000, Argentina.
  • Racca S; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Ciudad Universitaria, CC 242 Paraje El Pozo, Santa Fe 3000, Argentina.
  • Khawam JN; Grupo de Procesos Biológicos en Ingeniería Ambiental (GPBIA), Facultad de Ingeniería y Ciencias Hídricas (FICH), Universidad Nacional del Litoral (UNL), Ciudad Universitaria, CC 242 Paraje El Pozo, Santa Fe 3000, Argentina.
  • Leonardi RJ; Grupo de Procesos Biológicos en Ingeniería Ambiental (GPBIA), Facultad de Ingeniería y Ciencias Hídricas (FICH), Universidad Nacional del Litoral (UNL), Ciudad Universitaria, CC 242 Paraje El Pozo, Santa Fe 3000, Argentina.
  • Tomassi AH; Grupo de Procesos Biológicos en Ingeniería Ambiental (GPBIA), Facultad de Ingeniería y Ciencias Hídricas (FICH), Universidad Nacional del Litoral (UNL), Ciudad Universitaria, CC 242 Paraje El Pozo, Santa Fe 3000, Argentina.
  • Benzzo MT; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Ciudad Universitaria, CC 242 Paraje El Pozo, Santa Fe 3000, Argentina.
  • Comelli RN; Grupo de Procesos Biológicos en Ingeniería Ambiental (GPBIA), Facultad de Ingeniería y Ciencias Hídricas (FICH), Universidad Nacional del Litoral (UNL), Ciudad Universitaria, CC 242 Paraje El Pozo, Santa Fe 3000, Argentina.
J Ind Microbiol Biotechnol ; 512024 Jan 09.
Article em En | MEDLINE | ID: mdl-38936832
ABSTRACT
d-Xylose is a metabolizable carbon source for several non-Saccharomyces species, but not for native strains of S. cerevisiae. For the potential application of xylose-assimilating yeasts in biotechnological processes, a deeper understanding of pentose catabolism is needed. This work aimed to investigate the traits behind xylose utilization in diverse yeast species. The performance of 9 selected xylose-metabolizing yeast strains was evaluated and compared across 3 oxygenation conditions. Oxygenation diversely impacted growth, xylose consumption, and product accumulation. Xylose utilization by ethanol-producing species such as Spathaspora passalidarum and Scheffersomyces stipitis was less affected by oxygen restriction compared with other xylitol-accumulating species such as Meyerozyma guilliermondii, Naganishia liquefaciens, and Yamadazyma sp., for which increased aeration stimulated xylose assimilation considerably. Spathaspora passalidarum exhibited superior conversion of xylose to ethanol and showed the fastest growth and xylose consumption in all 3 conditions. By performing assays under identical conditions for all selected yeasts, we minimize bias in comparisons, providing valuable insight into xylose metabolism and facilitating the development of robust bioprocesses. ONE-SENTENCE

SUMMARY:

This work aims to expand the knowledge of xylose utilization in different yeast species, with a focus on how oxygenation impacts xylose assimilation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oxigênio / Xilose / Etanol / Fermentação Idioma: En Revista: J Ind Microbiol Biotechnol Assunto da revista: BIOTECNOLOGIA / MICROBIOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Argentina País de publicação: Alemanha
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oxigênio / Xilose / Etanol / Fermentação Idioma: En Revista: J Ind Microbiol Biotechnol Assunto da revista: BIOTECNOLOGIA / MICROBIOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Argentina País de publicação: Alemanha