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Short communication: Goat mastitis and the formation of neutrophil extracellular traps (NETs).
Caldeira, Jéssica Lobo Albuquerque; Costa, Daiene Gaione; Polveiro, Richard Costa; Gomes do Rêgo, Maria Eduarda; Barbosa, Wagner Faria; de Oliveira, Leandro Licursi; Moreira, Maria Aparecida Scatamburlo.
Afiliação
  • Caldeira JLA; Bacterial Diseases Laboratory, Department of Preventive Veterinary Medicine and Public Health, Veterinary Department, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
  • Costa DG; Bacterial Diseases Laboratory, Department of Preventive Veterinary Medicine and Public Health, Veterinary Department, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
  • Polveiro RC; Bacterial Diseases Laboratory, Department of Preventive Veterinary Medicine and Public Health, Veterinary Department, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
  • Gomes do Rêgo ME; Bacterial Diseases Laboratory, Department of Preventive Veterinary Medicine and Public Health, Veterinary Department, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
  • Barbosa WF; Department of Statistics, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
  • de Oliveira LL; Immunochemistry and Glycobiology Laboratory, Department of General Biology, Universidade Federal de Viçosa, University Campus, PH Rolfs Avenue, Viçosa, Minas Gerais 36570-000, Brazil.
  • Moreira MAS; Bacterial Diseases Laboratory, Department of Preventive Veterinary Medicine and Public Health, Veterinary Department, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil. Electronic address: masm@ufv.br.
Vet Immunol Immunopathol ; 274: 110793, 2024 Aug.
Article em En | MEDLINE | ID: mdl-38943998
ABSTRACT
Mastitis, an inflammation of the mammary gland affecting milk production and quality in dairy herds, is often associated with Staphylococcus spp. in goats. Neutrophils are crucial in combating infections by migrating into milk and deploying various defense strategies, including the release of neutrophil extracellular traps (NETs) composed of DNA, histones, and bactericidal proteins. This study investigated whether NETs are released by goat neutrophils stimulated in vitro by Staphylococcus aureus and Staphylococcus warneri, two common pathogens of goat mastitis. PMNs were isolated from blood from healthy adult goats. We evaluated goat NET formation by stimulating cells with phorbol 12-myristate 13-acetate (PMA) as a positive control, cytochalasin for inhibition of actin polymerization, S. aureus, and S. warneri. NET formation was observed in response to chemical stimulation and bacterial presence, effectively trapping pathogens. Variations in NET formation between S. aureus and S. warneri suggest pathogen-specific responses. These findings suggest that the formation of NETs may be an important complementary mechanism in the defense against mastitis in goats. In conclusion, this study unveils a novel defense mechanism in goats, indicating the role of NETs against S. aureus and S. warneri in mastitis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções Estafilocócicas / Staphylococcus aureus / Cabras / Doenças das Cabras / Armadilhas Extracelulares / Mastite / Neutrófilos Limite: Animals Idioma: En Revista: Vet Immunol Immunopathol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções Estafilocócicas / Staphylococcus aureus / Cabras / Doenças das Cabras / Armadilhas Extracelulares / Mastite / Neutrófilos Limite: Animals Idioma: En Revista: Vet Immunol Immunopathol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda