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Chemoprotective effect of arbutin on azoxymethane-induced aberrant crypt foci in rat colon via modulation of PCNA/Bax protein.
Ahmed, K A; Shareef, S H; Faraj, T A; Abdulla, M A; Najmaldin, S K; Agha, N F S; Kheder, R K.
Afiliação
  • Ahmed KA; Department of Medical Laboratory Sciences, Faculty of Allied Medical Sciences, Al-Ahliyya Amman University, Amman, Jordan.
  • Shareef SH; Department of Biology, College of Education, Salahaddin University-Erbil, Erbil, Kurdistan Region, Iraq.
  • Faraj TA; Department of Basic Sciences, College of Medicine, Hawler Medical University, Erbil, Iraq.
  • Abdulla MA; Department of Medical Analysis, Faculty of Applied Science, Tishk International University, Erbil, Kurdistan Region, Iraq.
  • Najmaldin SK; Department of Medical Analysis, Faculty of Applied Science, Tishk International University, Erbil, Kurdistan Region, Iraq.
  • Agha NFS; Department of Medical Analysis, Faculty of Applied Science, Tishk International University, Erbil, Kurdistan Region, Iraq.
  • Kheder RK; Department of Anesthesia, Erbil Medical Technical Institute, Erbil Polytechnic University, Erbil, Kurdistan Region, Iraq.
Braz J Med Biol Res ; 57: e13306, 2024.
Article em En | MEDLINE | ID: mdl-38958363
ABSTRACT
Arbutin is utilized in traditional remedies to cure numerous syndromes because of its anti-microbial, antioxidant, and anti-inflammatory properties. This study aimed to evaluate chemopreventive effects of arbutin on azoxymethane (AOM)-induced colon aberrant crypt foci (ACF) in rats. Five groups of rats were used normal control group (rats injected hypodermically with sterile phosphate-buffered saline once per week for two weeks) and groups 2-5, which were subcutaneously inoculated with 15 mg/kg AOM once a week for two weeks. AOM control and 5-fluorouracil (5-FU) control groups were fed 10% Tween orally daily for 8 weeks using a feeding tube. The treated groups were fed 30 and 60 mg/kg arbutin every day for 2 months. ACF from the AOM control group had aberrant nuclei in addition to multilayered cells and an absence of goblet cells. The negative control group displayed spherical cells and nuclei in basal positions. Histological examination revealed a reduced number of AFC cells from colon tissues of the 5-FU reference group. Arbutin-fed animals showed down-regulation of proliferating cell nuclear antigen (PCNA) and up-regulation of Bax protein compared to AOM control. Rats fed with arbutin displayed a significant increase of superoxide dismutase (SOD) and catalase (CAT) activities in colon tissue homogenates compared to the AOM control group. In conclusion, arbutin showed therapeutic effects against colorectal cancer, explained by its ability to significantly decrease ACF, down-regulate PCNA protein, and up-regulate Bax protein. In addition, arbutin significantly increased SOD and CAT, and decreased malondialdehyde (MDA) levels, which might be due to its anti-proliferative and antioxidant properties.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Arbutina / Azoximetano / Antígeno Nuclear de Célula em Proliferação / Proteína X Associada a bcl-2 / Focos de Criptas Aberrantes Limite: Animals Idioma: En Revista: Braz J Med Biol Res Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Jordânia País de publicação: Brasil
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Arbutina / Azoximetano / Antígeno Nuclear de Célula em Proliferação / Proteína X Associada a bcl-2 / Focos de Criptas Aberrantes Limite: Animals Idioma: En Revista: Braz J Med Biol Res Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Jordânia País de publicação: Brasil