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Loading of bacterial cellulose dressing with frutalin, a lectin from Artocarpus incisa L.
da Silva, Thamyres Freire; Leite, Talita Abrante; de Souza, Francisco Fábio Pereira; da Silva Barroso, Wallady; de Souza Guedes, Luciana; da Silva, André Luís Coelho; de Souza, Bartolomeu Warlene Silva; Vieira, Rodrigo Silveira; Andrade, Fábia Karine.
Afiliação
  • da Silva TF; Adsorption Separation Group, Department of Chemical Engineering, Federal University of Ceará, Fortaleza, Ceará 60455-760, Brazil.
  • Leite TA; Molecular and Structural Biotechnology Group, Department of Biochemistry and Biology, Federal University of Ceará, 60020-181 Fortaleza, Ceará, Brazil.
  • de Souza FFP; Adsorption Separation Group, Department of Chemical Engineering, Federal University of Ceará, Fortaleza, Ceará 60455-760, Brazil.
  • da Silva Barroso W; Molecular and Structural Biotechnology Group, Department of Biochemistry and Biology, Federal University of Ceará, 60020-181 Fortaleza, Ceará, Brazil.
  • de Souza Guedes L; Adsorption Separation Group, Department of Chemical Engineering, Federal University of Ceará, Fortaleza, Ceará 60455-760, Brazil.
  • da Silva ALC; Molecular and Structural Biotechnology Group, Department of Biochemistry and Biology, Federal University of Ceará, 60020-181 Fortaleza, Ceará, Brazil. Electronic address: andre.coelho@ufc.br.
  • de Souza BWS; Department of Fisheries Engineering, Federal University of Ceará, Fortaleza, Ceará 60455-970, Brazil.
  • Vieira RS; Adsorption Separation Group, Department of Chemical Engineering, Federal University of Ceará, Fortaleza, Ceará 60455-760, Brazil.
  • Andrade FK; Adsorption Separation Group, Department of Chemical Engineering, Federal University of Ceará, Fortaleza, Ceará 60455-760, Brazil. Electronic address: fabiakarine@gmail.com.
Int J Biol Macromol ; 276(Pt 2): 133774, 2024 Sep.
Article em En | MEDLINE | ID: mdl-39004244
ABSTRACT
Bacterial cellulose (BC), produced by bacterial fermentation, is a high-purity material. BC can be oxidized (BCOXI), providing aldehyde groups for covalent bonds with drugs. Frutalin (FTL) is a lectin capable of modulating cell proliferation and remodeling, which accelerates wound healing. This study aimed to develop an FTL-incorporated dressing based on BC, and to evaluate its physicochemical properties and biological activity in vitro. An experimental design was employed to maximize FTL loading yield onto the BC and BCOXI, where independent variables were FTL concentration, temperature and immobilization time. BCOXI-FTL 1 (44.96 % ± 1.34) had the highest incorporation yield (IY) at the experimental conditions 6 h, 5 °C, 20 µg mL-1. The second highest yield was BCOXI-FTL 6 (23.28 % ± 1.43) using 24 h, 5 °C, 100 µg mL-1. Similarly, the same reaction parameters provided higher immobilization yields for native bacterial cellulose BC-FTL 6 (16.91 % ± 1.05) and BC-FTL 1 (21.71 % ± 1.57). Purified FTL displayed no cytotoxicity to fibroblast cells (<50 µg mL-1 concentration) during 24 h. Furthermore, BCOXI-FTL and BC-FTL were non-cytotoxic during 24 h and stimulated fibroblast migration. BCOXI-FTL demonstrated neutrophil activation in vitro similar to FTL. These promising results indicate that the bacterial cellulose matrices containing FTL at low concentrations, could be used as an innovative biomaterial for developing wound dressings.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bandagens / Celulose / Artocarpus / Lectinas de Plantas Limite: Animals Idioma: En Revista: Int J Biol Macromol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bandagens / Celulose / Artocarpus / Lectinas de Plantas Limite: Animals Idioma: En Revista: Int J Biol Macromol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda