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Discovery of candidate biomarkers from plasma-derived extracellular vesicles of patients with cirrhosis and hepatocellular carcinoma: an exploratory proteomic study.
Zertuche-Martínez, Cecilia; Velázquez-Enríquez, Juan Manuel; González-García, Karina; Santos-Álvarez, Jovito Cesar; Romero-Tlalolini, María de Los Ángeles; Pina-Canseco, Socorro; Pérez-Campos Mayoral, Laura; Muriel, Pablo; Villa-Treviño, Saúl; Baltiérrez-Hoyos, Rafael; Arellanes-Robledo, Jaime; Vásquez-Garzón, Verónica Rocío.
Afiliação
  • Zertuche-Martínez C; Laboratorio de Fibrosis y cáncer, Facultad de Medicina y Cirugía, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca de Juárez 68120, Oaxaca, Mexico.
  • Velázquez-Enríquez JM; Laboratorio de Fibrosis y cáncer, Facultad de Medicina y Cirugía, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca de Juárez 68120, Oaxaca, Mexico.
  • González-García K; Laboratorio de Fibrosis y cáncer, Facultad de Medicina y Cirugía, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca de Juárez 68120, Oaxaca, Mexico.
  • Santos-Álvarez JC; Laboratorio de Fibrosis y cáncer, Facultad de Medicina y Cirugía, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca de Juárez 68120, Oaxaca, Mexico.
  • Romero-Tlalolini MLÁ; CONAHCYT-Facultad de Medicina y Cirugía, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca de Juárez 68120, Oaxaca, Mexico. vrvasquezga@conacyt.mx.
  • Pina-Canseco S; Centro de Investigación Facultad de Medicina UNAM UABJO, Facultad de Medicina y Cirugía, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca de Juárez 68120, Oaxaca, Mexico.
  • Pérez-Campos Mayoral L; Centro de Investigación Facultad de Medicina UNAM UABJO, Facultad de Medicina y Cirugía, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca de Juárez 68120, Oaxaca, Mexico.
  • Muriel P; Laboratorio de Hepatología Experimental, Departamento de Farmacología, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Ciudad de México 07000, Mexico.
  • Villa-Treviño S; Departamento de Biología Celular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Ciudad de México 07360, Mexico.
  • Baltiérrez-Hoyos R; CONAHCYT-Facultad de Medicina y Cirugía, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca de Juárez 68120, Oaxaca, Mexico. vrvasquezga@conacyt.mx.
  • Arellanes-Robledo J; CONAHCYT-Instituto Nacional de Medicina Genómica, Ciudad de México 14610, Mexico.
  • Vásquez-Garzón VR; CONAHCYT-Facultad de Medicina y Cirugía, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca de Juárez 68120, Oaxaca, Mexico. vrvasquezga@conacyt.mx.
Mol Omics ; 20(7): 483-495, 2024 Aug 12.
Article em En | MEDLINE | ID: mdl-39011654
ABSTRACT
Extracellular vesicles (EVs) represent an attractive source of biomarkers due to their biomolecular cargo. The aim of this study was to identify candidate protein biomarkers from plasma-derived EVs of patients with liver cirrhosis (LC) and hepatocellular carcinoma (HCC). Plasma-derived EVs from healthy participants (HP), LC, and HCC patients (eight samples each) were subjected to label-free quantitative proteomic analysis using LC-MS/MS. A total of 248 proteins were identified, and differentially expressed proteins (DEPs) were obtained after pairwise comparison. We found that DEPs mainly involve complement cascade activation, coagulation pathways, cholesterol metabolism, and extracellular matrix components. By choosing a panel of up- and down-regulated proteins involved in cirrhotic and carcinogenesis processes, TGFBI, LGALS3BP, C7, SERPIND1, and APOC3 were found to be relevant for LC patients, while LRG1, TUBA1C, TUBB2B, ACTG1, C9, HP, FGA, FGG, FN1, PLG, APOB and ITIH2 were associated with HCC patients, which could discriminate both diseases. In addition, we identified the top shared proteins in both diseases, which included LCAT, SERPINF2, A2M, CRP, and VWF. Thus, our exploratory proteomic study revealed that these proteins might play an important role in the disease progression and represent a panel of candidate biomarkers for the prognosis and diagnosis of LC and HCC.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biomarcadores Tumorais / Carcinoma Hepatocelular / Proteômica / Vesículas Extracelulares / Cirrose Hepática / Neoplasias Hepáticas Limite: Female / Humans / Male / Middle aged Idioma: En Revista: Mol Omics Ano de publicação: 2024 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biomarcadores Tumorais / Carcinoma Hepatocelular / Proteômica / Vesículas Extracelulares / Cirrose Hepática / Neoplasias Hepáticas Limite: Female / Humans / Male / Middle aged Idioma: En Revista: Mol Omics Ano de publicação: 2024 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido