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Osmotically Sensitive TREK Channels in Rat Articular Chondrocytes: Expression and Functional Role.
Ponce, Arturo; Ogazon Del Toro, Alejandro; Jimenez, Lidia; Roldan, Maria Luisa; Shoshani, Liora.
Afiliação
  • Ponce A; Department of Physiology, Biophysics and Neurosciences, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-IPN), Ciudad de México 07360, Mexico.
  • Ogazon Del Toro A; Department of Physiology, Biophysics and Neurosciences, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-IPN), Ciudad de México 07360, Mexico.
  • Jimenez L; Department of Physiology, Biophysics and Neurosciences, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-IPN), Ciudad de México 07360, Mexico.
  • Roldan ML; Department of Physiology, Biophysics and Neurosciences, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-IPN), Ciudad de México 07360, Mexico.
  • Shoshani L; Department of Physiology, Biophysics and Neurosciences, Center for Research and Advanced Studies of the National Polytechnic Institute (CINVESTAV-IPN), Ciudad de México 07360, Mexico.
Int J Mol Sci ; 25(14)2024 Jul 18.
Article em En | MEDLINE | ID: mdl-39063089
ABSTRACT
Articular chondrocytes are the primary cells responsible for maintaining the integrity and functionality of articular cartilage, which is essential for smooth joint movement. A key aspect of their role involves mechanosensitive ion channels, which allow chondrocytes to detect and respond to mechanical forces encountered during joint activity; nonetheless, the variety of mechanosensitive ion channels involved in this process has not been fully resolved so far. Because some members of the two-pore domain potassium (K2P) channel family have been described as mechanosensors in other cell types, in this study, we investigate whether articular chondrocytes express such channels. RT-PCR analysis reveals the presence of TREK-1 and TREK-2 channels in these cells. Subsequent protein expression assessments, including Western blotting and immunohistochemistry, confirm the presence of TREK-1 in articular cartilage samples. Furthermore, whole-cell patch clamp assays demonstrate that freshly isolated chondrocytes exhibit currents attributable to TREK-1 channels, as evidenced by activation by arachidonic acid (AA) and ml335 and further inhibition by spadin. Additionally, exposure to hypo-osmolar shock activates currents, which can be attributed to the presence of TREK-1 channels, as indicated by their inhibition with spadin. Therefore, these findings highlight the expression of TREK channels in rat articular chondrocytes and suggest their potential involvement in regulating the integrity of cartilage extracellular matrix.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cartilagem Articular / Condrócitos / Canais de Potássio de Domínios Poros em Tandem Limite: Animals Idioma: En Revista: Int J Mol Sci Ano de publicação: 2024 Tipo de documento: Article País de afiliação: México País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cartilagem Articular / Condrócitos / Canais de Potássio de Domínios Poros em Tandem Limite: Animals Idioma: En Revista: Int J Mol Sci Ano de publicação: 2024 Tipo de documento: Article País de afiliação: México País de publicação: Suíça