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Antimicrobial and anthelmintic effects of copper nanoparticles against Koi carp parasites and their toxicity.
Dos Santos, Gracienhe Gomes; Riofrio, Liseth Vanessa Perengue; Sousa, Ana Paula; Santos, Paola Capistrano; Silva, Alexandre Vaz; Lopes, Emilly Monteiro; Costa, Domickson Silva; Pereira Júnior, Jucimauro de Araújo; Ferreira, Giulia Beatrice; Silva, Graziella Vivine Gonçalves de Matos; Tedesco, Marilia; Nogueira, Diego José; Jerônimo, Gabriela Tomas; Martins, Mauricio Laterça.
Afiliação
  • Dos Santos GG; AQUOS-Aquatic Organisms Health Laboratory, Aquaculture Department, UFSC, Florianópolis, SC, Brazil.
  • Riofrio LVP; AQUOS-Aquatic Organisms Health Laboratory, Aquaculture Department, UFSC, Florianópolis, SC, Brazil.
  • Sousa AP; AQUOS-Aquatic Organisms Health Laboratory, Aquaculture Department, UFSC, Florianópolis, SC, Brazil.
  • Santos PC; AQUOS-Aquatic Organisms Health Laboratory, Aquaculture Department, UFSC, Florianópolis, SC, Brazil.
  • Silva AV; AQUOS-Aquatic Organisms Health Laboratory, Aquaculture Department, UFSC, Florianópolis, SC, Brazil.
  • Lopes EM; AQUOS-Aquatic Organisms Health Laboratory, Aquaculture Department, UFSC, Florianópolis, SC, Brazil.
  • Costa DS; AQUOS-Aquatic Organisms Health Laboratory, Aquaculture Department, UFSC, Florianópolis, SC, Brazil.
  • Pereira Júnior JA; AQUOS-Aquatic Organisms Health Laboratory, Aquaculture Department, UFSC, Florianópolis, SC, Brazil.
  • Ferreira GB; AQUOS-Aquatic Organisms Health Laboratory, Aquaculture Department, UFSC, Florianópolis, SC, Brazil.
  • Silva GVGM; AQUOS-Aquatic Organisms Health Laboratory, Aquaculture Department, UFSC, Florianópolis, SC, Brazil.
  • Tedesco M; AQUOS-Aquatic Organisms Health Laboratory, Aquaculture Department, UFSC, Florianópolis, SC, Brazil.
  • Nogueira DJ; LABCAI- Laboratory of Biomarkers of Aquatic Contamination and Immunochemistry, UFSC, Florianópolis, SC, Brazil.
  • Jerônimo GT; AQUOS-Aquatic Organisms Health Laboratory, Aquaculture Department, UFSC, Florianópolis, SC, Brazil.
  • Martins ML; AQUOS-Aquatic Organisms Health Laboratory, Aquaculture Department, UFSC, Florianópolis, SC, Brazil.
J Fish Dis ; : e14003, 2024 Jul 29.
Article em En | MEDLINE | ID: mdl-39075836
ABSTRACT
This study investigated the in vitro antimicrobial and anthelmintic effect of copper nanoparticles (CuNPs) against the bacterium Aeromonas hydrophila, the monogeneans Dactylogyrus minutus, Dactylogyrus extensus, Gyrodactylus cyprini, and the cestode Schyzocotyle acheilognathi, as well as their toxicity to Cyprinus carpio Koi. In the antimicrobial in vitro test, the inhibition zone method and minimum inhibitory concentration (MIC) were performed. In order to determine the time and efficacy of monogenean parasite mortality, the parasites were exposed to CuNP concentrations of 20, 50, 100, 150, 200, and 300 mg L-1, and a control group with tank water and one with copper sulphate pentahydrate (CuSO4.5H2O) at a concentration of 0.3 mg L-1, performed in triplicate. The parasites were observed every 10 min for 300 min, and mortality was recorded. For the cestodes, parasites were immersed in CuNP concentrations of 50, 100, 150, and 300 mg L-1. At the end of the in vitro tests, the anthelmintic efficacy of each treatment was calculated. To assess the tolerance and toxicity in fish, they were exposed to CuNP concentrations of 0.6, 1.25, 2.5, 5, 10, 20, and 50 mg L-1 for 12 h. The MIC demonstrated that CuNPs effectively inhibited the growth of A. hydrophila up to a dilution of 12,500 mg L-1 and showed an inhibition zone of 14.0 ± 1.6 mm for CuNPs. The results of anthelmintic activity showed a dose-dependent effect of concentration for both groups of parasites, with the most effective concentration being 300 mg L-1 in 120 min. In the toxicity test, the carps showed tolerance to lower concentrations. The study indicated that CuNPs were effective against the studied pathogens. However, it proved to be toxic to fish at high concentrations. The use of low concentrations is recommended still requires further investigation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: J Fish Dis Assunto da revista: BIOLOGIA / MEDICINA VETERINARIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: J Fish Dis Assunto da revista: BIOLOGIA / MEDICINA VETERINARIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido