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Synthetic hemozoin as a nanocarrier for cross-presentation.
Torres-Dias, Letícia; Souza, Rebeca Santana; Moreira, Jessica Carolina Alves; Paggi, Douglas de Oliveira; do Amaral, Jônatas Bussador; Bachi, André Luis Lacerda; Augusto, Leonardo; Shio, Marina Tiemi.
Afiliação
  • Torres-Dias L; Post-Graduation Program in Health Science, Santo Amaro University (UNISA), São Paulo, Brazil.
  • Souza RS; Graduation in Biomedicine, Santo Amaro University (UNISA), São Paulo, Brazil.
  • Moreira JCA; Graduation in Biomedicine, Santo Amaro University (UNISA), São Paulo, Brazil.
  • Paggi DO; Post-Graduation Program in Health Science, Santo Amaro University (UNISA), São Paulo, Brazil.
  • do Amaral JB; ENT Research Lab. Department of Otorhinolaryngology -Head and Neck Surgery, Federal University of Sao Paulo, Sao Paulo, Brazil.
  • Bachi ALL; Post-Graduation Program in Health Science, Santo Amaro University (UNISA), São Paulo, Brazil.
  • Augusto L; Department of Pathology, Microbiology, and Immunology. University of Nebraska Medical Center Omaha, United States of America.
  • Shio MT; Post-Graduation Program in Health Science, Santo Amaro University (UNISA), São Paulo, Brazil. Electronic address: mtshio@prof.unisa.br.
Immunobiology ; 229(5): 152837, 2024 Sep.
Article em En | MEDLINE | ID: mdl-39089130
ABSTRACT
It is known that conventional antigen presentation involves phagocytosis of antigens followed by its internalization in endocytic compartments and presentation of epitopes through MHC class II molecules for CD4 T cells. However, since 1976 a cross-presentation pathway has been studied, in which CD8 T cells are activated via MHC class I with antigens acquired through phagocytosis or endocytosis by dendritic cells (DCs). Among some important molecules involved in the cross-presentation, the C-type lectin receptor of the Dectin-1 cluster (CLECs), particularly the CLEC9A receptor, not only is expressed in dendritic cells but also presents a pivotal role in this context. In special, CLEC12A has been highlighted as a malaria pigment hemozoin (HZ) receptor. During Plasmodium infection, hemozoin crystals defend the parasite against heme toxicity within erythrocytes, as well as the released native HZ elicits pro-inflammatory responses and can induce cross-presentation. Particularly, this crystal can be synthesized from hematin anhydride and mimics the native form, and the gaps generated between the nanocrystal domains during its synthesis allow for substance coupling followed by its coating. Therefore, this study aimed to assess whether synthetic hemozoin (sHz) or hematin anhydride could be a nanocarrier and promote cross-presentation in dendritic cells. Firstly, it was verified that sHz can carry coated and coupled antigens, the compounds can associate to LAMP1-positive vesicles and decrease overall intracellular pH, which can potentially enhance the cross-presentation of ovalbumin and Leishmania infantum antigens. Thus, this study adds important data in the molecular intricacies of antigen presentation by showing not only the sHz immunomodulatory properties but also its potential applications as an antigen carrier.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células Dendríticas / Apresentação de Antígeno / Apresentação Cruzada / Hemeproteínas Limite: Animals / Humans Idioma: En Revista: Immunobiology Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células Dendríticas / Apresentação de Antígeno / Apresentação Cruzada / Hemeproteínas Limite: Animals / Humans Idioma: En Revista: Immunobiology Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda