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Impact of the near-physiological temperature on the in vitro maturation of bovine oocytes: A comparative proteomic approach.
Tavares, Winny Caldas Moreno; Maretto, Vinicius; Silveira, Vanildo; Pinto, Vitor Batista; Bustamante-Filho, Ivan Cunha; Quirino, Celia Raquel; Ortiz Vega, Wilder Hernando; Caldas-Bussiere, Maria Clara.
Afiliação
  • Tavares WCM; Laboratory of Animal Reproduction and Breeding, State University of Norte Fluminense 'Darcy Ribeiro' (Universidade Estadual do Norte Fluminense Darcy Ribeiro - UENF), Avenida Alberto Lamego, 2000, Parque Califórnia, Campos dos Goytacazes, Rio de Janeiro, RJ, 28013-602, Brazil.
  • Maretto V; Laboratory of Animal Reproduction and Breeding, State University of Norte Fluminense 'Darcy Ribeiro' (Universidade Estadual do Norte Fluminense Darcy Ribeiro - UENF), Avenida Alberto Lamego, 2000, Parque Califórnia, Campos dos Goytacazes, Rio de Janeiro, RJ, 28013-602, Brazil.
  • Silveira V; Laboratório de Biotecnologia, Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Av. Alberto Lamego 2000, Campos dos Goytacazes, RJ, 28013-602, Brazil; Unidade de Biologia Integrativa, Setor de Genômica e Proteômica, UENF, Campos dos Goytacaz
  • Pinto VB; Laboratório de Biotecnologia, Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Av. Alberto Lamego 2000, Campos dos Goytacazes, RJ, 28013-602, Brazil; Unidade de Biologia Integrativa, Setor de Genômica e Proteômica, UENF, Campos dos Goytacaz
  • Bustamante-Filho IC; Laboratório de Biotecnologia da Reprodução Animal, Universidade do Vale do Taquari - Univates, Rua Avelino Tallini, 171, Lajeado, RS, 95914-014, Brazil.
  • Quirino CR; Laboratory of Animal Reproduction and Breeding, State University of Norte Fluminense 'Darcy Ribeiro' (Universidade Estadual do Norte Fluminense Darcy Ribeiro - UENF), Avenida Alberto Lamego, 2000, Parque Califórnia, Campos dos Goytacazes, Rio de Janeiro, RJ, 28013-602, Brazil.
  • Ortiz Vega WH; Laboratory of Animal Reproduction and Breeding, State University of Norte Fluminense 'Darcy Ribeiro' (Universidade Estadual do Norte Fluminense Darcy Ribeiro - UENF), Avenida Alberto Lamego, 2000, Parque Califórnia, Campos dos Goytacazes, Rio de Janeiro, RJ, 28013-602, Brazil.
  • Caldas-Bussiere MC; Laboratory of Animal Reproduction and Breeding, State University of Norte Fluminense 'Darcy Ribeiro' (Universidade Estadual do Norte Fluminense Darcy Ribeiro - UENF), Avenida Alberto Lamego, 2000, Parque Califórnia, Campos dos Goytacazes, Rio de Janeiro, RJ, 28013-602, Brazil. Electronic address: cl
Theriogenology ; 228: 64-74, 2024 Oct 15.
Article em En | MEDLINE | ID: mdl-39098122
ABSTRACT
In vivo, the temperature inside preovulatory follicles of cows is approximately 1 °C lower than rectal temperature. However, standard bovine oocyte in vitro maturation (IVM) protocols use 38.5 °C based on rectal temperature. This study evaluated the effect of reducing IVM temperature to 37.5 °C on the proteomic profile of oocytes compared to the routine 38.5 °C. Nuclear maturation rate and cumulus cell (CC) expansion (30 COCs per group, 21 replicates) were assessed by observing the first polar body and using a subjective scoring method (0-4). Total nitrite concentrations in the culture medium were measured using the Griess method. Differential proteomics was performed using LC-MS/MS on pooled oocyte samples (500 matured oocytes per group, three replicates), followed by gene ontology enrichment, protein-protein interaction, and putative miRNA target analyses. No significant differences were observed between the groups in nuclear maturation, CC expansion, or nitrite concentration (P > 0.05). A total of 806 proteins were identified, with 7 up-regulated and 12 down-regulated in the treatment group compared to the control. Additionally, 12 proteins were unique to the control group, and 8 were unique to the treatment group. IVM at 37.5 °C resulted in the upregulation of proteins involved in protein folding and GTP binding, and the downregulation of enzymes with oxidoreductase activity and proteins involved in cytoskeletal fiber formation. Furthermore, 43 bovine miRNAs potentially regulating these genes (DES, HMOX2, KRT75, FARSA, IDH2, CARHSP1) were identified. We conclude that IVM of bovine oocytes at 37.5 °C induces significant proteomic changes without impacting nuclear maturation, cumulus cell expansion, or nitrite concentration in the IVM medium.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oócitos / Proteômica / Técnicas de Maturação in Vitro de Oócitos Limite: Animals Idioma: En Revista: Theriogenology Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oócitos / Proteômica / Técnicas de Maturação in Vitro de Oócitos Limite: Animals Idioma: En Revista: Theriogenology Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos