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Chemical gasification: An alternative approach to in vitro maturation of bovine oocytes.
Gómez-López, David L; Velasco-Acosta, Diego A; Chávez-Rodríguez, Aldemar; Schneider, Augusto; Rocha, Juan F; Dubeibe-Marín, Diego F.
Afiliação
  • Gómez-López DL; Colombian Corporation of Agricultural Research (Corporación Colombiana de Investigación Agropecuaria - AGROSAVIA), Research Center Tibaitatá, Mosquera, Colombia.
  • Velasco-Acosta DA; Colombian Corporation of Agricultural Research (Corporación Colombiana de Investigación Agropecuaria - AGROSAVIA), Research Center Tibaitatá, Mosquera, Colombia.
  • Chávez-Rodríguez A; Private Practice, Bogotá, Colombia.
  • Schneider A; Federal University of Pelotas (Universidade Federal de Pelotas), Pelotas, RS, Brazil.
  • Rocha JF; Colombian Corporation of Agricultural Research (Corporación Colombiana de Investigación Agropecuaria - AGROSAVIA), Research Center Tibaitatá, Mosquera, Colombia.
  • Dubeibe-Marín DF; Faculty of Agricultural Sciences, University of Applied and Environmental Sciences (Universidad de Ciencias Aplicadas y Ambientales - UDCA), Bogotá, Colombia.
Reprod Domest Anim ; 59(8): e14701, 2024 Aug.
Article em En | MEDLINE | ID: mdl-39109447
ABSTRACT
This study aimed to evaluate the effect of chemical gasification and HEPES as alternative systems to pH control during in vitro maturation on bovine oocytes competence. Groups of 20 bovine cumulus oocytes complexes (COCs) were randomly distributed and cultured for 24 h in one of the following experimental groups (i) chemical reaction (ChRG) system CO2 generated from sodium bicarbonate and citric acid reaction (ii) culture media TCM-HEPES (HEPES-G); and (iii) control group (CNTG) in conventional incubator. After in vitro maturation (IVM), the COCs were in vitro fertilized (IVF), and in vitro cultivated (IVC) in a conventional incubator. We evaluated oocyte nuclear maturation, cleavage and blastocyst rates, in addition to the relative mRNA expression of BAX, BMP-15, AREG and EREG genes in oocytes and cumulus cells. The proportion of oocytes in metaphase II was higher in CNTG and ChRG (77.57% and 77.06%) than in the HEPES-G (65.32%; p = .0408 and .0492, respectively). The blastocyst production was similar between CNTG and ChRG (26.20% and 28.47%; p = .4232) and lower (p = .001) in the HEPES-G (18.71%). The relative mRNA expression of BAX gene in cumulus cells was significantly higher (p = .0190) in the HEPES-G compared to the CNTG. Additionally, the relative mRNA expression of BMP-15 gene was lower (p = .03) in oocytes from HEPES-G compared to the CNTG. In conclusion, inadequate atmosphere control has a detrimental effect on oocyte maturation. Yet, the use of chemical gasification can be an efficient alternative to bovine COCs cultivation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oócitos / Fertilização in vitro / Técnicas de Maturação in Vitro de Oócitos Limite: Animals Idioma: En Revista: Reprod Domest Anim Assunto da revista: MEDICINA REPRODUTIVA / MEDICINA VETERINARIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Alemanha
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oócitos / Fertilização in vitro / Técnicas de Maturação in Vitro de Oócitos Limite: Animals Idioma: En Revista: Reprod Domest Anim Assunto da revista: MEDICINA REPRODUTIVA / MEDICINA VETERINARIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Alemanha