Your browser doesn't support javascript.
loading
Unlocking Biological Activity and Metabolomics Insights: Primary Screening of Cyanobacterial Biomass from a Tropical Reservoir.
Médice, Rhuana Valdetário; Arruda, Renan Silva; Yoon, Jaewon; Borges, Ricardo Moreira; Noyma, Natália Pessoa; Lürling, Miquel; Crnkovic, Camila Manoel; Marinho, Marcelo Manzi; Pinto, Ernani.
Afiliação
  • Médice RV; Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, Brazil.
  • Arruda RS; Department of Plant Biology, Rio de Janeiro State University, Rio de Janeiro, RJ, Brazil.
  • Yoon J; Department of Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, Brazil.
  • Borges RM; Walter Mors Natural Product Research Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Noyma NP; Department of Plant Biology, Rio de Janeiro State University, Rio de Janeiro, RJ, Brazil.
  • Lürling M; Department of Environmental Sciences, Wageningen University, Wageningen, The Netherlands.
  • Crnkovic CM; Department of Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, Brazil.
  • Marinho MM; Department of Plant Biology, Rio de Janeiro State University, Rio de Janeiro, RJ, Brazil.
  • Pinto E; Center for Nuclear Energy in Agriculture, University of São Paulo, Piracicaba, SP, Brazil.
Environ Toxicol Chem ; 2024 Aug 07.
Article em En | MEDLINE | ID: mdl-39110011
ABSTRACT
Cyanobacterial harmful algal blooms can pose risks to ecosystems and human health worldwide due to their capacity to produce natural toxins. The potential dangers associated with numerous metabolites produced by cyanobacteria remain unknown. Only select classes of cyanopeptides have been extensively studied with the aim of yielding substantial evidence regarding their toxicity, resulting in their inclusion in risk management and water quality regulations. Information about exposure concentrations, co-occurrence, and toxic impacts of several cyanopeptides remains largely unexplored. We used liquid chromatography-mass spectrometry (LC-MS)-based metabolomic methods associated with chemometric tools (NP Analyst and Data Fusion-based Discovery), as well as an acute toxicity essay, in an innovative approach to evaluate the association of spectral signatures and biological activity from natural cyanobacterial biomass collected in a eutrophic reservoir in southeastern Brazil. Four classes of cyanopeptides were revealed through metabolomics microcystins, microginins, aeruginosins, and cyanopeptolins. The bioinformatics tools showed high bioactivity correlation scores for compounds of the cyanopeptolin class (0.54), in addition to microcystins (0.54-0.58). These results emphasize the pressing need for a comprehensive evaluation of the (eco)toxicological risks associated with different cyanopeptides, considering their potential for exposure. Our study also demonstrated that the combined use of LC-MS/MS-based metabolomics and chemometric techniques for ecotoxicological research can offer a time-efficient strategy for mapping compounds with potential toxicological risk. Environ Toxicol Chem 2024;001-10. © 2024 SETAC.
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Environ Toxicol Chem Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Environ Toxicol Chem Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos