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Recombinant proteins as promising antigens applied to the immunodiagnosis of Chagas disease: a scoping review.
Resende, Carlos Ananias Aparecido; Ribeiro, Anna Júlia; Gandra, Isadora Braga; Silva, Kamila Alves; Lopes, Lucas da Silva; Barcelos, Isabelle Caroline Dos Santos; Couto, Carolina Alves Petit; de Faria, Mariana Teixeira; Pereira, Sabrina Paula; Xavier, Sandra Rodrigues; Machado, Juliana Martins; da Paz, Mariana Campos; Chaves, Ana Thereza; Coelho, Eduardo Antônio Ferraz; Giunchetti, Rodolfo Cordeiro; Chávez-Fumagalli, Miguel Angel; Dutra, Walderez Ornelas; Gonçalves, Ana Alice Maia; Galdino, Alexsandro Sobreira.
Afiliação
  • Resende CAA; Microorganism Biotechnology Laboratory, National Institute of Science and Technology on Industrial Biotechnology (INCT-BI), Federal University of São João Del-Rei, Divinópolis, Brazil.
  • Ribeiro AJ; Microorganism Biotechnology Laboratory, National Institute of Science and Technology on Industrial Biotechnology (INCT-BI), Federal University of São João Del-Rei, Divinópolis, Brazil.
  • Gandra IB; Microorganism Biotechnology Laboratory, National Institute of Science and Technology on Industrial Biotechnology (INCT-BI), Federal University of São João Del-Rei, Divinópolis, Brazil.
  • Silva KA; Microorganism Biotechnology Laboratory, National Institute of Science and Technology on Industrial Biotechnology (INCT-BI), Federal University of São João Del-Rei, Divinópolis, Brazil.
  • Lopes LDS; Microorganism Biotechnology Laboratory, National Institute of Science and Technology on Industrial Biotechnology (INCT-BI), Federal University of São João Del-Rei, Divinópolis, Brazil.
  • Barcelos ICDS; Microorganism Biotechnology Laboratory, National Institute of Science and Technology on Industrial Biotechnology (INCT-BI), Federal University of São João Del-Rei, Divinópolis, Brazil.
  • Couto CAP; Microorganism Biotechnology Laboratory, National Institute of Science and Technology on Industrial Biotechnology (INCT-BI), Federal University of São João Del-Rei, Divinópolis, Brazil.
  • de Faria MT; Microorganism Biotechnology Laboratory, National Institute of Science and Technology on Industrial Biotechnology (INCT-BI), Federal University of São João Del-Rei, Divinópolis, Brazil.
  • Pereira SP; Microorganism Biotechnology Laboratory, National Institute of Science and Technology on Industrial Biotechnology (INCT-BI), Federal University of São João Del-Rei, Divinópolis, Brazil.
  • Xavier SR; Microorganism Biotechnology Laboratory, National Institute of Science and Technology on Industrial Biotechnology (INCT-BI), Federal University of São João Del-Rei, Divinópolis, Brazil.
  • Machado JM; Microorganism Biotechnology Laboratory, National Institute of Science and Technology on Industrial Biotechnology (INCT-BI), Federal University of São João Del-Rei, Divinópolis, Brazil.
  • da Paz MC; Laboratory of Bioactives and Nanobiotechnology, Federal University of São João Del-Rei, Divinópolis, Brazil.
  • Chaves AT; Postgraduate Program in Health Sciences, Infectious Diseases and Tropical Medicine, Faculty of Medicine, Federal University of Minas Gerais, Belo Horizonte, Brazil.
  • Coelho EAF; Postgraduate Program in Health Sciences, Infectious Diseases and Tropical Medicine, Faculty of Medicine, Federal University of Minas Gerais, Belo Horizonte, Brazil.
  • Giunchetti RC; Laboratory of Biology of Cell Interactions, Department of Morphology, National Institute of Science and Technology on Tropical Diseases (INCT-T), Federal University of Minas Gerais, Belo Horizonte, Brazil.
  • Chávez-Fumagalli MA; Computational Biology and Chemistry Research Group, Vicerrectorado de Investigación, Universidad Católica de Santa María, Arequipa, Peru.
  • Dutra WO; Laboratory of Biology of Cell Interactions, Department of Morphology, National Institute of Science and Technology on Tropical Diseases (INCT-T), Federal University of Minas Gerais, Belo Horizonte, Brazil.
  • Gonçalves AAM; Microorganism Biotechnology Laboratory, National Institute of Science and Technology on Industrial Biotechnology (INCT-BI), Federal University of São João Del-Rei, Divinópolis, Brazil.
  • Galdino AS; Microorganism Biotechnology Laboratory, National Institute of Science and Technology on Industrial Biotechnology (INCT-BI), Federal University of São João Del-Rei, Divinópolis, Brazil.
Front Microbiol ; 15: 1420226, 2024.
Article em En | MEDLINE | ID: mdl-39139374
ABSTRACT
Chagas disease (CD), caused by the protozoan Trypanosoma cruzi, is an important public health problem, occurring mainly in Latin America. The disease has a major social and economical effect, negatively impacting the life of the infected individuals, and bringing great costs to public health. An early and accurate diagnosis is essential for administration of early treatment. In addition, prognostic tests may aid disease management, decreasing hospitalization costs. However, the serological diagnostic scenario for CD still faces several challenges, making the development of new diagnostic kits a pressing matter. Facing this scenario, several researchers have expanded efforts in developing and testing new antigens, such as recombinant proteins and recombinant multiepitope proteins, with promising results. These recombinant antigens offer several advantages, such as improved sensitivity and specificity, in addition to facilitated scaling. Also, it has been possible to observe a rising number of studies using ELISA and point-of-care platforms, employing these antigens in the past few years. Among them, recombinant proteins were the most applied antigens, demonstrating great capacity to discriminate between positive and negative samples. Although fewer in number, recombinant multiepitope proteins also demonstrated an improved diagnostic performance. Indeed, a great number of studies employing these antigens showed sensitivity and specificity values above 90%, greatly impacting diagnostic accuracy. Nevertheless, despite the good results found, it is still possible to observe some bottlenecks in the development of new antigens, such as the scarcity of tests with sera from the acute phase and the variability of results in different geographic areas. In this sense, aiming to contribute to control and health programs, the continuous search for a more accurate serological diagnosis is essential, both for the acute and chronic phases of the disease.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Front Microbiol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Front Microbiol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça