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Ultrasound as a safe and reliable guidance for subarachnoid puncture in rabbits
Pereira, Ivan Dias Fernandes; Vital, Roberto Bezerra; Silva, Ronaldo; Valerini, Felipe Gilberto; Machado, Vania Maria de Vasconcelos; Marques, Mariangela Esrher; Miot, Helio; Navarro, Lais Helena; Ganem, Eliana Marisa.
Afiliação
  • Pereira, Ivan Dias Fernandes; Universidade Estadual Paulista. Department of Anesthesiology. Botucatu. Brazil
  • Vital, Roberto Bezerra; Universidade Estadual Paulista. Department of Anesthesiology. Botucatu. Brazil
  • Silva, Ronaldo; Universidade Estadual Paulista. Department of Anesthesiology. Botucatu. Brazil
  • Valerini, Felipe Gilberto; Universidade Estadual Paulista. Botucatu. Brazil
  • Machado, Vania Maria de Vasconcelos; Universidade Estadual Paulista. Department of Animal Reproduction and Veterinary Radiology. Botucatu. Brazil
  • Marques, Mariangela Esrher; Universidade Estadual Paulista. Department of Pathology. Botucatu. Brazil
  • Miot, Helio; Universidade Estadual Paulista. Department of Dermatology. Botucatu. Brazil
  • Navarro, Lais Helena; Universidade Estadual Paulista. Department of Anesthesiology. Botucatu. Brazil
  • Ganem, Eliana Marisa; Universidade Estadual Paulista. Department of Anesthesiology. Botucatu. Brazil
Acta cir. bras. ; 32(1): 14-21, Jan. 2017. ilus, tab
Article em En | VETINDEX | ID: vti-15560
Biblioteca responsável: BR68.1
Localização: BR68.1
ABSTRACT

Purpose:

To evaluate a model for studying the toxicity in nervous tissue and meninges using ultrasound to guide needle insertion into the subarachnoid space of rabbits, with the objective of avoiding injuries triggered by the puncture and by intraneural injection of solutions.

Methods:

Forty-five adult female rabbits were divided into 3 groups (G) G1 underwent subarachnoid puncture, G2 underwent subarachnoid injection of saline solution and G3 underwent subarachnoid injection of 0.5% hyperbaric bupivacaine. A needle was inserted into the S1-S2 subarachnoid space guided by ultrasound. The sensitivity and motility of the animals were evaluated for 3 days, after which the animals were sacrificed for removal of lumbar and sacral portions of the spinal cord for histological examination by light microscopy and immunohistochemistry.

Results:

All animals had motor function and pain sensitivity on the evaluation period. No animal had complication during or after the procedures.

Conclusion:

Ultrasound is a useful and safe method for the correct identification of the subarachnoid space of rabbits.(AU)
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Texto completo: 1 Base de dados: VETINDEX Assunto principal: Punção Espinal / Espaço Subaracnóideo / Toxicidade / Raquianestesia / Tecido Nervoso Limite: Animals Idioma: En Revista: Acta cir. bras. Ano de publicação: 2017 Tipo de documento: Article
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google

Texto completo: 1 Base de dados: VETINDEX Assunto principal: Punção Espinal / Espaço Subaracnóideo / Toxicidade / Raquianestesia / Tecido Nervoso Limite: Animals Idioma: En Revista: Acta cir. bras. Ano de publicação: 2017 Tipo de documento: Article