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Identification and validation of novel EST-SSR markers in olives
Arbeiter, Alenka Baruca; Hladnik, Matjaz; Jakse, Jernej; Bandelj, Dunja.
Afiliação
  • Arbeiter, Alenka Baruca; University of Primorska. Faculty of Mathematics, Natural Sciences and Information Technologies. Koper. SI
  • Hladnik, Matjaz; University of Primorska. Faculty of Mathematics, Natural Sciences and Information Technologies. Koper. SI
  • Jakse, Jernej; University of Ljubljana. Biotechnical Faculty. Agronomy Dept. Ljubljana. SI
  • Bandelj, Dunja; University of Primorska. Faculty of Mathematics, Natural Sciences and Information Technologies. Koper. SI
Sci. agric ; 74(3): 215-225, mai./jun. 2017. tab, ilus, graf
Article em En | VETINDEX | ID: biblio-1497638
Biblioteca responsável: BR68.1
Localização: BR68.1
ABSTRACT
The olive (Olea europaea L.) is a leading oil crop in the Mediterranean area. Limited information on the inheritance of agronomic significant traits hinders progress in olive breeding programs, which encourages the development of markers linked to the traits. In this study, we report on the development of 46 olive simple sequence repeat (SSR) markers, obtained from 577,025 expressed sequence tags (ESTs) in developing olive fruits generated in the framework of the Slovenian national olive transcriptome project. Sequences were de novo assembled into 98,924 unigenes, which were then used as a source for microsatellites searching. We identified 923 unigenes that contained 984 SSRs among which dinucleotide SSRs (36 %) were the most abundant, followed by tri- (33 %) and hexa- (21 %) nucleotides. Microsatellite repeat motif GA (37 %) was the most common among dinucleotides, while microsatellite repeat motif GAA was the most abundant trinucleotide SSR motif (16 %). Gene ontology annotations could be assigned to 27 % of the unigenes. A hundred and ten expressed sequence tag-derived-simple sequence repeats (EST-SSRs) with annotated genes were selected for primer designing and finally, 46 (42 %) polymorphic EST-SSRs were successfully amplified and used to validate genetic diversity among 24 olive varieties. The average number of alleles per locus, observed heterozygosity, expected heterozygosity, and polymorphic information content were 4.5, 0.649, 0.604 and 0.539, respectively. Twenty-seven EST-SSRs showed good diversity properties and were recommended for further olive genome investigation.
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Texto completo: 1 Base de dados: VETINDEX Assunto principal: Marcadores Genéticos / Genoma de Planta / Etiquetas de Sequências Expressas / Olea Idioma: En Revista: Sci. agric / Sci. agric. Ano de publicação: 2017 Tipo de documento: Article
Texto completo
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Texto completo: 1 Base de dados: VETINDEX Assunto principal: Marcadores Genéticos / Genoma de Planta / Etiquetas de Sequências Expressas / Olea Idioma: En Revista: Sci. agric / Sci. agric. Ano de publicação: 2017 Tipo de documento: Article