RESUMO

Using synthetic peptides and a combinatorial library of 56 mer random oligonucleotides, we have developed reagents that behave as "synthetic antibodies". The results obtained with the protein phosphatase 2A as a model system are shown here. The specificity of these reagents, named "oligobodies", has been demonstrated by Western blot analysis and immunohistochemistry. The oligobodies have enormous advantages compared to antibodies: their production is independent of the immune system, they can be prepared in a few days and there is no need for a purified target protein. These reagents can be produced even if the corresponding protein was never isolated or purified, since only a partial DNA sequence from a database provides enough information to make them.

Assuntos

Especificidade de Anticorpos , Oligonucleotídeos/síntese química , Biblioteca de Peptídeos , Animais , Western Blotting , Imuno-Histoquímica , Camundongos , Camundongos Endogâmicos C57BL , Oligonucleotídeos/imunologia , Fosfoproteínas Fosfatases/imunologia , Reação em Cadeia da Polimerase , Proteína Fosfatase 2 , Coelhos , Análise de Sequência de DNA

RESUMO

Using synthetic peptides and a combinatorial library of 56 mer random oligonucleotides, we have developed reagents that behave as [quot ]synthetic antibodies[quot ]. The results obtained with the protein phosphatase 2A as a model system are shown here. The specificity of these reagents, named [quot ]oligobodies[quot ], has been demonstrated by Western blot analysis and immunohistochemistry. The oligobodies have enormous advantages compared to antibodies: their production is independent of the immune system, they can be prepared in a few days and there is no need for a purified target protein. These reagents can be produced even if the corresponding protein was never isolated or purified, since only a partial DNA sequence from a database provides enough information to make them.