RESUMO
Serum samples from 894 wild animals (representing 31 species) from Trinidad and Grenada were examined by the microscopic agglutination test for leptospiral antibodies; 198 were positive. These included 39 bats, 88 mongooses, six opossums, 10 peridomestic rodents, 15 forest rodents, 10 lizards, and 30 toads. Thirteen pathogenic serogroups were involved. Thirty-nine Leptospira isolates were reported from mongooses, opossums, rodents and toads.
Assuntos
Grupos de População Animal , Animais Selvagens , Reservatórios de Doenças/veterinária , Leptospirose/veterinária , Aglutininas/análise , Animais , Anticorpos Antibacterianos/análise , Leptospira/imunologia , Leptospira/isolamento & purificação , Leptospirose/microbiologia , Especificidade da Espécie , Trinidad e Tobago , Índias OcidentaisRESUMO
During outbreaks of dengue fever in the Caribbean in 1977 and 1978 a continuous cell line derived from Aedes pseudoscutellaris was successfully used for the isolation of dengue virus strains from acute human sera. 238 strains were isolated and culture fluid was successfully used as antigen for the identification of several strains. The isolates all produced a marked syncytial cytopathic effect often visible as early as four days after inoculation. The method was successfully employed in the field where, because of their low optimal incubation temperature, the cells suffered no ill effects under ambient conditions. The isolation method was found to be much more sensitive than conventional mouse inoculation.
Assuntos
Vírus da Dengue/isolamento & purificação , Dengue/microbiologia , Aedes/microbiologia , Animais , Sangue/microbiologia , Linhagem Celular , Humanos , Métodos , Índias OcidentaisRESUMO
The yellow fever epidemic in Trinidad began with an epizootic in Alouatta monkeys in November, 1978. Activity was detected by virus isolation from Alouatta monkeys and from Haemagogus mosquitoes between this date and July 1980. First reports of monkey deaths centered around the Guayaguayare forests of SOuth Trinidad, following which the epizootic spread west to the Moruga area, and north through the Biche Forest Reserve, eventually reaching the north-west Chaguaramas peninsula. Surveillance of febrile persons attending clinics and hospitals was intensified and 18 confirmed cases were identified, from 14 of whom yellow fever virus was isolated. In four cases, diagnoses were made on the basis of liver pathology only. Paired sera were obtained from 10 of 11 persons who survived and in each case serological conversions were obtained by haemaglutination inhibition and more neutralization tests. Yellow fever virus was isolated from 16 of 32 Alouatta monkeys and from 19 of 174 pools of Haemagogus mosquitoes. Two systems were used for virus isolation: the Aedes cell line AP-61 and suckling mice. The former proved to be more sensitive, detecting virus in 11.7 percent of 725 specimens inoculated whereas suckling mice detected virus in 7.9 percent of 724 specimens. In 11 instances virus was detected in an animal or mosquito pool by AP-61 but not initially by mouse inoculation whereas the converse was true in only one case (AU)