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Pseudomonas aeruginosa can produce pigments, which mediate external electron transfer (EET). Depending on the mediator, this species can be explored in bioelectrosystems to harvest energy or to obtain chemicals from residual organic compounds. This study has compared the performance of microbial fuel cells (MFCs) inoculated with a Pseudomonas aeruginosa isolate, namely EW603 or EW819, which produce pyocyanin and pyoverdine, respectively. The efficiency of these MFCs in glycerol, a typical residue of biodiesel production, were also compared. The MFCs exhibited different performances. The maximum voltage was 411 and 281 mV m2, the power density was 40.1 and 21.3 mW m-2, and the coulombic efficiency was 5.16 and 1.49% for MFC-EW603 and MFC-EW819, respectively. MFC-EW603 and MFC-EW819 achieved maximum current at 560 and 2200 Ω, at 141.2 and 91.3 mA m-2, respectively. When the system was operated at the respective maximum current output, MFC-EW603 consumed the total glycerol content (11 mmol L-1), and no products could be detected after 50 h. In turn, acetic and butyric acids were detected at the end of MFC-EW819 operation (75 h). The results suggested that P. aeruginosa metabolism can be steered in the MFC to generate current or microbial products depending on the pigment-producing strain and the conditions applied to the system, such as the external resistance. In addition, gene cluster pathways related to phenazine production (phzA and phzB) and other electrogenic-related genes (mexGHI-opmB) were identified in the strain genomes, supporting the findings. These results open new possibilities for using glycerol in bioelectrochemical systems.
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Fontes de Energia Bioelétrica , Piocianina/metabolismo , Pseudomonas aeruginosa/genética , Pseudomonas aeruginosa/metabolismo , Pseudomonas , Glicerol/metabolismo , Eletrodos , EletricidadeRESUMO
Tejuino, is a Mexican fermented beverage prepared by germination-fermentation or nixtamalization-fermentation (artisanal and commercial mode respectively) of maize. The aim of this study was to evaluate the gut metabolites, volatile, and phenolic compounds (PC) produced by the indigestible fraction (IF) of Tejuino during an in vitro colonic fermentation. Twenty-six PC in the IF were identified; the hydroxycinnamic acids (30-40 %) were the most abundant. In the IF of Tejuino pyrogallol, and urolithins were identified. Some of the representative PC of maize as maysin derivatives (apimaysin and 3-methoxymaysin) (flavonoids). The quantification of acetic and butyric acid become notable after 6 h of the colonic fermentation of IF of Tejuino. Ninety-seven volatile compounds were found, and the PCA shows the predominant compounds as short chain fatty acids, esters of organic acids and indole derivatives. These results suggest that Tejuino could be an important source of metabolites with high biological value.
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Flavorings are widely used in food and beverage industries and spray drying is the most cost-effective encapsulation technique to deliver stable products. Generally, the same slurry is used to encapsulate both hydrophilic and hydrophobic flavors which led sometimes to lower retention. The same slurry formulation composed by Modified Starch and Maltodextrin 20DE was loaded with 35% of two different flavorings (orange and passion fruit) and, spray dried under the same conditions. The flavorings selected had different octanol/water partition coefficients and their composition affected the emulsion stability. Orange flavoring presented clearly better emulsion stability than passion fruit flavoring, confirmed by size distribution and Turbiscan Stability Index (TSI orange ⪠TSI passion fruit). A key learning from this work is that the best infeed emulsion achieved by the most hydrophobic flavoring, presented the lowest droplet size and yielded in final bigger particle size and the best encapsulation efficiency result (>92%).
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INTRODUCTION: The kisspeptin gene Kiss1 is expressed in two hypothalamic areas: anteroventral periventricular nucleus/periventricular nucleus (AVPV/PeN) and arcuate nucleus (ARC), and also in gonads. Several pieces of evidence suggests that gamma-amino butyric acid B receptors (GABAB) signaling can regulate Kiss1 expression. Here, we inhibited GABAB signaling from PND2 to PND21 and evaluated the hypothalamic-pituitary-gonadal (HPG) axis. METHODS: BALB/c mice were treated on postnatal days 2-21 (PND2-PND21) with CGP55845 (GABAB antagonist) and evaluated in PND21 and adulthood: gene expression (qPCR) in the hypothalamus and gonads, hormones by radioimmunoassay, gonad histochemistry (H&E), puberty onset, and estrous cycles. RESULTS: At PND21, CGP inhibited Kiss1 and Tac2 and increased Pdyn and Gabbr1 in the ARC of both sexes and decreased Th only in female AVPV/PeN. Serum follicle-stimulating hormone (FSH) and testis weight were decreased in CGP-males, and puberty onset was delayed. In adults, Kiss1, Tac2, Pdyn, Pgr, Cyp19a1, and Gad1 were downregulated, while Gabbr1 was upregulated in the ARC of both sexes. In the AVPV/PeN, Kiss1, Th, Cyp19a1, and Pgr were decreased while Gad1 was increased in CGP-females, whereas Cyp19a1 was increased in CGP-males. Serum FSH was increased in CGP-males while prolactin was increased in CGP-females. Testosterone and progesterone were increased in ovaries from CGP-females, in which Kiss1, Cyp19a1, and Esr1 were downregulated while Hsd3b2 was upregulated, together with increased atretic and decreased ovulatory follicles. Testes from CGP-males showed decreased progesterone, increased Gabbr1, Kiss1, Kiss1r, and Esr2 and decreased Cyp19a1, and clear signs of seminiferous tubules atrophy. CONCLUSION: These results demonstrate that appropriate GABAB signaling during this critical prepubertal period is necessary for the normal development of the HPG axis.
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Kisspeptinas , Progesterona , Animais , Núcleo Arqueado do Hipotálamo/metabolismo , Feminino , Hormônio Foliculoestimulante , Antagonistas GABAérgicos , Gônadas , Hipotálamo/metabolismo , Kisspeptinas/genética , Kisspeptinas/metabolismo , Masculino , Camundongos , Progesterona/metabolismo , Prolactina/metabolismo , Receptores de Kisspeptina-1/metabolismo , Maturidade Sexual/fisiologia , Testosterona/metabolismo , DesmameRESUMO
The objective was to evaluate the inclusion of chemical additives or bacterial inoculant in corn silage. The experimental design was completely randomized with five treatments: silage without additive; silage added with urea (3.0%); silage with limestone (3.0%); silage added with crystal sugar (3.0%); and silage with a bacterial inoculant. Five of them were opened during the fermentation process (15 days) to measure pH, and three were opened 60 days after ensiling to evaluate the ammonia nitrogen and organic acids content. For the pH at the end of the fermentation process, higher values were found for the limestone additive, followed by the silage with urea, which had higher participation of lactic acid, 19.06 ppm for limestone and 18.95 ppm for urea. Higher concentrations of acetic acid were observed in inoculant silages (18.49ppm) or silage without additive (18.46ppm). The ammonia nitrogen content was higher in the silage with urea (23.74mg dL-1), followed by the silage without additive (7.54mg dL-1), which also had the highest concentration of butyric acid (4.19ppm). The use of additives reduced the concentration of butyric acid in the silage. The bacterial inoculant was the most efficient in decreasing the pH of the ensiled material.
Objetivou-se avaliar a inclusão de aditivos químicos ou inoculante bacteriano na silagem de milho. O delineamento experimental foi inteiramente ao acaso, com cinco tratamentos: silagem sem aditivo; silagem aditivada com ureia (3,0%); silagem aditivada com calcário (3,0%); silagem aditivada com açúcar cristal (3,0%); e silagem com inoculante bacteriano. Cinco sacos de silagem foram abertos durante o processo fermentativo (15 dias) para mensuração de pH, e três 60 dias após a ensilagem, para avaliação do teor de nitrogênio amoniacal e de ácidos orgânicos. Para pH no final do processo fermentativo, maiores valores foram verificados para o aditivo calcário, seguido da silagem com ureia, os quais apresentaram maior participação de ácido lático, 19,06 ppm para calcário e 18,95 ppm para ureia. Maiores concentrações de ácido acético foram observadas nas silagens inoculante (18,49 ppm) ou sem aditivo (18,46 ppm). O teor de nitrogênio amoniacal foi superior na silagem com ureia (23,74mg dL '), seguida pela silagem sem aditivo (7,54mg dL), que também apresentou maior concentração de ácido butírico (4,19 ppm). O uso de aditivos reduziu a concentração de ácido butírico na silagem. O inoculante bacteriano foi mais eficiente que aditivos químicos no declínio do pH do material ensilado.
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Silagem/microbiologia , Ácido Acético/análise , Ácido Láctico/análise , Ácido Butírico/análise , Inoculantes Agrícolas , Amônia/administração & dosagemRESUMO
Butyric acid has been studied and utilized intensively in broiler chicken production in order to substitute growth-promoting antibiotics. However, the comprehensive literature on this topic makes it difficult to understand overall results, since there is a noticeable number of studies with conflicting conclusions. Although several research studies have shown that butyric acid may increment broiler chicken's performance, several other studies show the opposite. This work aimed to organize information to understand, through a meta-analysis, whether butyric acid can be used as a performance-enhancing additive for broiler chickens, and whether it can replace growth-promoting antibiotics. It was clear from the results that the effect of butyric acid depends on the microbiological challenge that broilers receive. When broilers are raised without health challenge, butyric acid and even antibiotics do not influence growth performance. However, when broilers are challenged, butyric acid provided an increase in weight gain and a significant improvement in feed conversion, matching the results of antibiotics. We conclude that butyric acid improves broiler performance and can be used to replace growth-promoting antibiotics.(AU)
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Animais , Galinhas/fisiologia , Ácido Butírico/análise , Aditivos Alimentares/química , Metanálise em Rede , Revisão SistemáticaRESUMO
Bioactive compounds from food products made from natural ingredients such as corn and common bean could target the NLRP3 inflammasome, protein scaffolds with a key role in the moderation of intestinal inflammation. This research aimed to evaluate the anti-inflammatory effect from the fermented non-digestible fraction of baked corn and common bean snack (FNDF), and its main components, on the modulation of NLRP3 inflammasome markers in vitro. For this, a THP-1 macrophage/differentiated Caco-2 cell co-culture was used as a model of intestinal inflammation. A disease control (DC) (LPS/human IFN-γ, 10 ng/mL) was compared with FNDF (40-300 µg/mL) and its pure components: gallic (38.85 µM) and butyric acids (6 µM), verbascose (0.06 µM), their mixture, and an anti-inflammatory control (tofacitinib, 5 µM). Compared to DC, FNDF (40 µg/mL) reduced the 48 h-basolateral nitrites (40-60%), IL-1ß/IL-18, and TNF-α production. Additionally, it decreased the total reactive oxygen species (36.3%) and nitric oxide synthase (6.9%) activities, increasing superoxide dismutase (228.2%) activity. Compared to NLRP3 positive control, FNDF components decreased NLRP3 markers (caspase-1 activity, IL-1ß, and apoptosis). These results highlight NLRP3-anti-inflammatory effects from FNDF components. This is the first report of the NLRP3 inflammasome modulation by digested food matrix components, using a co-culture approach.
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Ácido Butírico/farmacologia , Ácido Gálico/farmacologia , Inflamassomos/efeitos dos fármacos , Inflamação/tratamento farmacológico , Enteropatias/tratamento farmacológico , Proteína 3 que Contém Domínio de Pirina da Família NLR/metabolismo , Biomarcadores/metabolismo , Células CACO-2 , Diferenciação Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Técnicas de Cocultura , Citocinas/genética , Citocinas/metabolismo , Ácido Gálico/administração & dosagem , Regulação da Expressão Gênica/efeitos dos fármacos , Humanos , L-Lactato Desidrogenase/metabolismo , Lipopolissacarídeos/toxicidade , Monócitos , Proteína 3 que Contém Domínio de Pirina da Família NLR/genética , Óxido Nítrico Sintase , Nitritos/metabolismo , Superóxido DismutaseRESUMO
BACKGROUND: Salep is obtained by grinding dried orchid tubers and used as a valuable ingredient in the food industry. Because of the glucomannan content of salep, it is thought to have prebiotic potential. However, there is little information in studies concerning the fermentation characteristics and potential prebiotic properties of salep. The objective of this study was to investigate the effect of salep on bifidobacterial growth by measuring the highest optical density (OD), calculating the specific growth rates, and determining the production of lactic acid and short-chain fatty acids (acetic, propionic, and butyric acid) as a result of bacterial fermentation. RESULT: The OD and pH values obtained in this study showed that salep was utilized as a source of assimilable carbon and energy by the Bifidobacterium species (BS). All Bifidobacterium strains produced lactic, acetic, propionic, and butyric acid, indicating that salep is readily fermented by these bacteria. Salep at 1% (w/v) showed a similar effect on bifidobacterial growth as that promoted by 1% (w/v) glucose used as a traditional carbon source. CONCLUSIONS: Bifidobacterium species can develop in media containing salep as well as in glucose and exhibit the potential to be used as new sources of prebiotics.
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Pós/metabolismo , Bifidobacterium/crescimento & desenvolvimento , Bifidobacterium/metabolismo , Ácidos Graxos Voláteis/biossíntese , Propionatos/análise , Propionatos/metabolismo , Indústria Alimentícia , Ácido Acético/análise , Ácido Acético/metabolismo , Ácido Láctico/análise , Ácido Láctico/metabolismo , Probióticos , Ácido Butírico/análise , Ácido Butírico/metabolismo , Ácidos Graxos Voláteis/análise , Prebióticos , Fermentação , Concentração de Íons de HidrogênioRESUMO
Stevia is an important non-caloric sweetener that has health-beneficial properties. The objective is to evaluate growth, development, and rooting of stevia plants during different seasons of the year using growth hormones. Eight experiments were set up in Ciudad Guzman, Jalisco, Mexico, with three treatments (T): T1, indol-3 butyric acid (IBA) 7.4 mM; T2, alphanaphthylacetamide (ANA) 6.4 mM + IBA 0.3 mM; and T3, control. The variables evaluated were rooted plantlets, plant height, root length, number of leaves, stem diameter, leaf dry weight, stem dry weight, root dry weight, leaf area, shoot biomass, total biomass, as well as development and growth indexes. Four samplings were conducted in each experiment. The results show that the most appropriate months for propagating stevia cuttings are February, March, April, May, and July, when 96% to 99% of the cuttings rooted. The hormones had the best results related to production of root development. The control was outstanding only in variables related to production of shoot biomass and not to root development. It is concluded that stevia can be propagated vegetatively using cuttings treated with IBA 7.4 mM or ANA 6.4 mM + IBA 0.3 mM, preferable in the period from February to July, with the exception of June.
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We ensiled different levels of okara and ground corn to evaluate the effects on the fermentative pattern, aerobic stability, and chemical composition of resulting silages. The experimental design was completely randomized with four replicates per treatment. The okara levels were (dry matter basis): control (without okara) and 200, 300, 400, and 500 g kg−1 okara, with four replicates per treatment. Control silage did not contain okara, but water was added to adjust the moisture content (400 g kg−1 as fed). Mixtures were ensiled in lab-scale silos and stored for 150 days. Compared with the control silage, okara inclusion linearly increased crude protein (from 89.1 to 251 g kg−1 DM), ether extract (from 39.6 to 136 g kg−1 DM), neutral detergent fiber (from 79.9 to 174 g kg−1 DM), acid detergent fiber (from 22.4 to 119 g kg−1 DM), and ash (from 12.2 to 32.4 g kg−1 DM), whereas decreased dry matter content and in vitro dry matter digestibility (from 830 to 730 g kg−1 DM). The use of okara linearly increased lactic acid concentration but also intensified secondary fermentation. On the other hand, aerobic stability of silages increased due to okara inclusion because of the higher amount of short-chain fatty acids, such as butyric and acetic acids, which accumulated during fermentation. Okara inclusion in corn grain silage must be conditioned to the dry matter content at ensiling, but must not exceed 200 g kg−1 on dry matter basis.(AU)